| 工程菌及其固定化细胞对有机磷农药的降解 |
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| 引用本文： | 闫艳春,姚良同,宋晓妍,贾乐,周波,李风华.工程菌及其固定化细胞对有机磷农药的降解[J].中国环境科学,2001,21(5):0-0. |
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| 作者姓名： | 闫艳春 姚良同 宋晓妍 贾乐 周波 李风华 |
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| 作者单位： | 1. 山东农业大学生命科学学院,
2. 北京环境科学生物工程公司, |
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| 基金项目： | 山东省自然科学基金资助项目(Y98D16064) |
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| 摘 要： | 用抗性库蚊酯酶基因,引入原核表达载体pRL439,转化大肠杆菌HB101细胞,获得表达.通过酶切、Southern杂交鉴定重组质粒.研究了重组菌酯酶的活性,重组质粒pRL-B1表达的酯酶具有高酶活并能高效降解酯酶的特异性底物a-乙酸萘酯(a-NA)和β-乙酸萘酯(β-NA);经对重组菌进行细胞固定化后降解有机磷农药对硫磷(1605),反应时间短,降解效率高.
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| 关 键 词： | 对硫磷 工程菌 降解农药 细胞固定化 基因表达 |
| 文章编号： | 1000-6923(2001)05-0412-05 |
| 收稿时间： | 1900-01-01; |
| 修稿时间： | 2001年2月16日 |
Degradation of organic phserhorus pesticides by engineered bacteria and theci immobilized cells. |
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| YAN Yan-chun,YAO Liang-tong,SONG Xiao-yan,JIA Le,ZHOU Bo,LI Feng-hua.Degradation of organic phserhorus pesticides by engineered bacteria and theci immobilized cells.[J].China Environmental Science,2001,21(5):0-0. |
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| Authors: | YAN Yan-chun YAO Liang-tong SONG Xiao-yan JIA Le ZHOU Bo LI Feng-hua |
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| Institution: | YAN Yan-chun1,YAO Liang-tong1,SONG Xiao-yan1,JIA Le1,ZHOU Bo1,LI Feng-hua2 |
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| Abstract: | Through resistant esterase gene cloned into the vector pRL439 the expression had been done for E.coli on bacillius HB101 cell transformation. This recombinant plasmid pRLB1 was identified by digestion and Southern analysis. The activity of recombinant esterase was studied. The esterase expressed by pRLB1 possessed high enzyme activity and could effectively degrade the specific substrate a-naphthyl acetate (a-NA) and b-naphthyl(b-NA) of esterase. The recombinant engineered bacterium was immobilized. Assays showed that the organic phosphorus pesticide parathion (1605) was degraded by the immobilized cell within short time and high efficiency. |
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| Keywords: | parathion engineered bacteria degradation of pesticide immobilized cell gene expression |
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