黄孢原毛平革菌降解磷酸三苯酯的性能和机理

以白腐真菌的中的黄孢原毛平革菌（Phanerochaete chrysosporium）为实验菌种，考察不同环境条件因素对P.chrysosporium降解磷酸三苯酯（TPhP）的影响以及TPhP降解过程中菌体细胞特性的变化.结果表明，当孢子液的接种量为4%（V/V），葡萄糖浓度为5g/L，pH值为5~6时，经过6d的处理P.chrysosporium对5mg/L TPhP的降解率可达到60%以上.菌体的细胞色素P450酶在TPhP的降解转化过程中发挥了重要的作用，抑制P450酶的活性会导致TPhP降解率下降.在降解反应的前期，为加快TPhP的代谢转化，菌体胞内蛋白含量以及ATP酶活性出现明显升高.在TPhP胁迫下，菌体SOD和CAT的活性随降解反应的进行呈现先升高后降低的趋势，2种抗氧化酶协同作用维持TPhP降解过程中菌体胞内的氧化还原平衡.

The performance and mechanism of triphenyl phosphate biodegradation by Phanerochaete chrysosporium

Phanerochaete chrysosporium, one typical species of white rot fungi, was chosen as the experimental strains to investigate the effect of environmental condition on TPhP biodegradation by P. chrysosporium and the changes of cellular characteristics of P. chrysosporium during TPhP biodegradation. The results showed that the biodegradation efficiency of 5mg/L TPhP by P. chrysosporium was above 60% when inoculum size was 4% (V/V), pH was adjusted to 5~6 and initial glucose concentration was 5g/L. Cytochrome P450 of P. chrysosporium played an important role in TPhP biodegradation, the inhibition of cytochrome P450 could result in the decrease of TPhP biodegradation efficiency. In the earlier stage of TPhP biodegradation, the intracellular protein content and ATPase activity of P. chrysosporium increased remarkably to promote the metabolism and biotransformation of TPhP. Under the stress of TPhP, SOD and CAT activities of P. chrysosporium increased firstly and then decreased. There was a synergistic action between these two antioxidant enzymes in maintaining the intracellular redox balance during TPhP biodegradation.