超导强磁场对小麦抗氧化酶的诱导表达

研究了7T超导稳态强磁场在不同处理时间条件下对小麦幼苗的抗氧化系统(SOD、CAT活性和MDA含量)的影响,并对不同金属辅基SOD酶的诱导表达模式进行了探讨.结果表明,强磁场诱导了T-SOD活性和CAT酶活性的提高,在峰值之前SOD和CAT的活性随着剂量的增大逐渐提高,但随着强度的增加和处理时间的延长,酶活激活作用会有所下降.低剂量下,T-SOD活性的提高来自于CuZn-SOD的活性的提高,中、高剂量下,主要诱导Mn-SOD的活性提高.低场强和短时间的磁场处理能使MDA的含量降低,但随着处理时间的延长和磁场强度的增加,MDA含量逐渐升高.各种酶在不同的处理条件下,其酶活的变化峰值处于3~5T之间.

Induction of antioxidative enzymes in wheat exposed to high static magnetic fields (SMFs)

The effects of superconducting SMFs up to 7-tesla on antioxidation system of wheat seedling (SOD, CAT activity and MDA content) and the expression pattern of SOD isoenzyme with different metal prosthetic groups were investigated. Increasing total SOD activity and CAT activity were induced by HSMFs. These activities were enhanced gradually with magnetic flux density and reached the highest at 3 and 5T under 1 and 5h exposing time, respectively. The enhancement of T-SOD activity under low intensity was mainly due to the increase of CuZn-SOD activity, while at the moderate and high intensity, the increase of T-SOD was mainly attributed to the high expression of Mn-SOD activity. Malondialdehyde (MDA) content varied apparently with different magnetic flux density, showing that it decreased at low density and shorter treating time, but increased gradually with prolonging treat time and increasing magnetic flux density. Under different treating conditions, the peak values of antioxidative enzymes varied between 3~5T.