壬基酚聚氧乙烯醚降解前后的激素效应和诱变活性

采用重组基因酵母和SOS/Umu试验研究了壬基酚聚氧乙烯醚(NP₁₀EO)经生物降解前后的雌激素活性和致突变活性.结果表明,在降解反应开始后的4d内降解产物的雌激素活性较低且不具有致突变性.随着降解时间的不断增加,降解产物的雌激素活性和致突变性不断增强.降解20d后,降解产物的雌激素活性相当于1nmol 17-β雌二醇的61.6%,诱变比值大于2,表明壬基酚聚氧乙烯醚的生物降解产物同时具有雌激素活性和致突变活性,且两者在产生时间和活性数值方面均是同步的和成正相关的.因此致突变活性很可能是此类降解产物对生物机体具有雌激素活性的基础.

Estrogenic activities and mutation effects of nonylphenol ethoxylates before and after biodegradation

The estrogenic activities and mutation effects of nonylphenol ethoxylates before and after microbial degradation were studied with the recombinant gene yeast and SOS/Umu test. The products in 4 days after beginning of the degradation had lower estrogenic activities and no mutation effects; and the estrogenic activities and mutation effects were strengthened continuously with the degradation time. After 20 days of degradation, the estrogenic activities of the products were 61.6% of 1nmol E2 and the mutation ratios were bigger than 2. The microbial degradation of nonylphenol ethoxylates possessed simultaneously estrogenic activities and mutation effects, both of which were synchronous and related positively in production time and activities values; thus, mutation effects might be the basis of organism possessing estrogenic activities with these degradation products.