首页 | 本学科首页   官方微博 | 高级检索  
     检索      

生物表面活性剂逆胶束中漆酶催化性能的研究
引用本文:彭子原,袁兴中,彭馨,黄华军,崔凯龙,赵艳鸽.生物表面活性剂逆胶束中漆酶催化性能的研究[J].中国环境科学,2013,33(6):1091-1096.
作者姓名:彭子原  袁兴中  彭馨  黄华军  崔凯龙  赵艳鸽
作者单位:湖南大学环境科学与工程学院,湖南长沙410082;湖南大学环境生物与控制教育部重点实验室,湖南长沙410082
摘    要:对一种新型生物表面活性剂逆胶束中漆酶的催化性能进行了研究.以鼠李糖脂(RL,阴离子生物表面活性剂)为表面活性剂,异辛烷和正己醇为溶剂和助表面活性剂,构建逆胶束,分别考察了有机相组成、RL浓度、逆胶束体系含水率W0、缓冲溶液酸碱度及盐浓度等因素对该逆胶束体系中漆酶催化性能的影响.结果表明,当逆胶束有机相正己醇/异辛烷体积比为1:1.8,RL浓度为13mmol/L,体系含水率W0为42,缓冲溶液pH5.0,KCl浓度为10mmol/L时,该体系中漆酶具有较好的活性及稳定性.对比试验显示,与化学表面活性剂AOT逆胶束相比,RL逆胶束能使漆酶表现出较高的活性及稳定性,说明与传统化学表面活性剂相比生物表面活性剂在构建逆胶束酶体系上的具有一定的优越性.

关 键 词:鼠李糖脂  逆胶束  漆酶  催化性能  
收稿时间:2012-10-23;

Catalytic performance of laccase in a biosurfactant reversed micellar system
PENG Zi-yuan , YUAN Xing-zhong , PENG Xin , HUANG Hua-jun , CUI Kai-long , ZHAO Yan-ge.Catalytic performance of laccase in a biosurfactant reversed micellar system[J].China Environmental Science,2013,33(6):1091-1096.
Authors:PENG Zi-yuan  YUAN Xing-zhong  PENG Xin  HUANG Hua-jun  CUI Kai-long  ZHAO Yan-ge
Abstract:The catalytic performance of laccase in a novel reverse micellar system, which adopted rhamnolipid (RL, an anionic bio-surfactant) as surfactant with isooctane and hexanol as solvent and cosurfactant, were studied. The effect of several significant factors on the catalytic performance of laccase, such as the construction of organic phase, the concentration of RL, water content(W0), pH value and salt concentration, were investigated and analyzed. The optimum catalytic activity and stability of laccase could be approached at following condition: V(hexanol)/V(isooctane): 1:1.8, RL concentration: 13 mmol/L, W0: 42, pH: 5.0, KCl concentration: 10 mmol/L. For a comparative purpose, the catalytic performance of laccase in AOT-based reverse micellar system( AOT-RMS) and RL-based reverse micellar system( RL-RMS) were compared. Both of laccase’s catalytic activity and stability in RL-RMS were much higher than those in AOT-RMS, which unveiled that bio-surfactants were much more suitable in contrusting enzyme-reverse micellar systems compared with traditional synthetic surfactants.
Keywords:rhamnolipid  reverse micellar system  laccase  catalytic performance
本文献已被 万方数据 等数据库收录!
点击此处可从《中国环境科学》浏览原始摘要信息
点击此处可从《中国环境科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号