活性炭固定耐冷菌对硝基苯的降解特性

研究了用颗粒活性炭固定耐冷菌(Pseudomonas putida)对硝基苯降解的强化作用.结果表明,细菌被活性炭固定后,对硝基苯的降解能力提高.降解浓度为200mg/L的硝基苯所用时间由固定前的34h左右缩短为29.5h.浓度为400mg/L的硝基苯,对细菌有强烈抑制作用,细菌无法生长,但固定化细菌可直接降解浓度为400mg/L的硝基苯,且降解浓度为600mg/L的硝基苯只需46h.固定化细菌可连续4次降解浓度为400mg/L以下的硝基苯,但随着次数增多,降解速率减慢.用SEM扫描200mg/L硝基苯降解末期的生物活性炭的结果表明,Pseudomonas putida菌是降解过程中唯一的细菌.

Biodegradation of nitrobenzene by a strain immobilized on granular activated carbon

Effects of nitrobenzene biodegradation enhanced by Pseudomonas putida immobilized on granular activated carbon (GAC) was studied. The biodegradation time was shortened for 200mg/L nitrobenzene from approximately 34h to 29.5h with biological activated carbon (BAC) present. Nitrobenzene with concentration at 400mg/L could not been directly biotransformed by the bacteria due to the intense inhibition before the bacteria was immobilized within GAC. However, nitrobenzene at the same concentration was biodegraded in 46h by the BAC. In addition, BAC was reused for nitrobenzene biodegradation for 4 times with a slowly decreased degradation efficiency. At the end of biodegradation, the BAC at the final stage of 200mg/L nitrobenzene degradation was scanned by SEM. And Pseudomonas putida was the only bacteria effective for the biodegradation.