不同驯化方式实现SBR中部分反硝化的对比研究

部分反硝化（NO₃^--N→NO₂^--N）是一种非常具有前景的硝酸盐废水处理方法，其可以结合厌氧氨氧化实现低碳源、低能耗脱氮.本文以葡萄糖为碳源，对比研究3种部分反硝化驯化方式：R1（SBR缺氧）、R2（SBR缺氧-好氧交替运行）、R3（SBR缺氧搅拌伴随低曝气）.经过120d驯化后，对三种反应器污泥进行沿程实验，探索3种反硝化规律.结果显示引入溶解氧驯化后的R2、R3污泥相比R1能够更好地实现部分反硝化，且R3最佳.采用高通量测序技术，对运行109d时R1，R2，R3进行微生物群落结构对比分析.结果显示在有溶解氧参与的R2，R3反应器中的优势菌属为Candidatus Saccharibacteria，相对丰度分别为45.44%和34.96%，这是该菌首次作为反硝化污泥优势菌属被报道.另外R1反应器中微生物多样性要远大于R2，R3，这也从侧面说明R2，R3的反硝化菌更专性.初始pH值对R3驯化污泥影响的批试实验表明，初始pH值对亚硝酸盐积累有明显影响，且初始pH值越高，亚硝酸盐积累越多.

Comparative study on achieving partial denitrification in a SBR by different acclimation methods

The partial denitrification (NO₃^--N→NO₂^--N), a promising way for nitrate wastewater treatment, could combine with ANAMMOX technology to achieve nitrogen removal with low concentration of carbon source and poor energy. Three different domestication pattern, R1 (SBR under anoxic condition), R2 (SBR under anoxia-aerobic alternating condition) and R3 (SBR under anoxic condition with low-intensity aeration), were conducted using glucose as electron donor to find out the rule of nitrite accumulation in denitrification process. These three reactors achieved steady nitrite accumulation after 120-days run, and the sludge were taken, respectively, to explore the denitrification pattern of these three acclimatizing ways. The findings revealed that, compared with R1, the R2 and R3 sludge could better achieve partial denitrification due to the involving of dissolved oxygen. And R3 was the best way. The microbial community structure of R1, R2 and R3 sludges on the 109th day were analyzed and compared by high throughput sequencing. The results showed that the dominant bacteria were Candidatus Saccharibacteria in both R2 and R3 reactor, which were with the participation of dissolved oxygen, and the relative abundance was 45.44% and 34.96%, respectively. This was the first time that the Candidatus Saccharibacteria was reported as the dominant bacteria in the denitrifying sludge. Besides, the microbial diversity of R1reactor was much larger than that of R2 and R3, which indicated that the denitrifying bacteria in R2 and R3 reactors were more exclusive. The batch experiments showed that the initial pH had a significant effect on the accumulation of nitrite. The higher the pH indicated the higher accumulation rate of nitrite.