林可霉素菌渣堆肥抗生素抗性基因变化分析

为了研究抗生素菌渣堆肥过程中抗生素抗性基因（ARGs）的变化情况，以林可霉素菌渣-糠醛渣堆肥为研究对象，以污泥-糠醛渣堆肥为对照.运用荧光定量PCR技术检测到了堆肥过程中lnuA-01、sul1、ermA、ermB、ermC等5种林可霉素抗性基因和整合子基因intI1的变化情况.结果表明，堆肥化处理可以降解99%的林可霉素残留，两者堆肥ARGs总量绝对丰度均有较大增加，而相对丰度降低5%~22%.同时发现林可霉素菌渣堆肥有助于intI1的富集，表明林可霉素菌渣堆肥存在生态风险.冗余分析显示，ARGs变化受环境因子影响严重，影响顺序为pH值 > 林可霉素残留 > 温度 > C/N.

Variations of Antibiotic resistance genes during lincomycin mycelia dreg composting

In order to investigate the variation of antibiotic resistance genes during the antibiotic mycelia dregs composting process, five antibiotic resistance genes (lnuA-01, sul1, ermA, ermB, ermC) and one horizontal gene transfer (intI1) were detected from the compost by quantitative PCR technique. The results showed that 99% lincomycin was degraded after composting. For both the lincomycin mycelia dreg compost and sewage sludge compost, the absolute total of ARGs was all increased. By contrast, the relative total of ARGs were all decreased by 5% and 22% respectively. IntI1 was enriched in lincomycin mycelia dreg compost indicating the potential ecological risk. The redundancy analysis revealed that the antibiotic resistance genes were greatly influenced by the environmental factors and the order was pH > licomycin residue > temperature > C/N.