AOB与AnAOB在不同生物填料上挂膜效果的研究

针对厌氧氨氧化工艺运行过程中污泥流失严重与启动时间长等问题，本研究通过对多种市售生物填料的挂膜实验，筛选适合好氧氨氧化菌与厌氧氨氧化菌挂膜的生物填料.结果表明，当活性污泥中的好氧氨氧化菌菌属为Nitrosomonas时，AQ1聚氨酯立方体填料最适合其挂膜，挂膜成熟时好氧氨氧化速率可以达到（0.81±0.08）mg N/（L·h），挂膜生物量为（0.87±0.14）mg VSS.而更适合厌氧氨氧化菌（Candidatus Kuenenia）挂膜的生物填料为K3环形填料，材质为聚乙烯或者聚丙烯.当厌氧氨氧化菌挂膜成熟时，其厌氧氨氧化速率可以达到（3.27±0.10）mg N/（L·h），挂膜生物量为（2.74±0.40）mg VSS.厌氧氨氧化菌在K3型填料上的挂膜要优于好氧氨氧化菌，其原因是厌氧氨氧化菌分泌的胞外聚合物含量要高于好氧氨氧化菌，而胞外聚合物是形成生物膜的重要因素.

Biofilm formation of ammonia-oxidizing bacteria and anaerobic ammonium-oxidizing bacteria on different biocarries

The typical setbacks of the operation of anaerobic ammonium oxidation (Anammox) process were the serious sludge loss and long startup time. Herein, the biofilm formation experiments were designed to select several commercial biocarriers with better biofilm performance for ammonia-oxidizing bacteria (AOB) and anaerobic ammonium-oxidizing bacteria (AnAOB). The results showed that AQ1polyurethane cubic biocarrier was the most suitable biocarrier for AOB to form biofilm. The ammonia oxidation rate of matured biofilm was (0.81±0.08)mg N/(L·h) and the biofilm biomass was (0.87±0.14)mg VSS. Nitrosomonas was dominant species in the activated sludge. For AnAOB (Candidatus Kuenenia), K3 annular biocarrier (made of polyethylene or polypropylene) was the most suitable biocarrier for the biofilm formation. The anaerobic ammonium oxidation rate of matured biofilm could reach (3.27±0.10)mg N/(L·h), and the biofilm biomass was (2.74±0.40)mg VSS. The biofilm formation of AnAOB on the K3 biocarrier was better than AOB. The reason was that the content of extracellular polymeric substances secreted by AnAOB was higher than that of AOB, and extracellular polymeric substances play an important role in biofilm formation.