游离亚硝酸预处理对剩余污泥电解及微生物群落结构的影响

为打破传统厌氧发酵周期长，有机质利用率低等瓶颈，增强污泥的资源利用和能源回收，探讨了游离亚硝酸（FNA）预处理对剩余污泥电解效果及微生物群落的影响.对比分析了FNA预处理前后剩余污泥在微生物电解池（MEC）中的电流和氢气产生、溶解性有机物和挥发酸的释放和利用及功能菌群的变化情况.结果表明，FNA预处理能有效地促进剩余污泥在MEC系统中的水解和酸化，其溶解性糖类、蛋白和挥发酸的含量远高于未预处理组，进而促进了水解发酵菌、产电菌及反硝化菌的生长和富集，最终挥发酸利用率均在97%以上，表现为电流（1.9mA）和氢气（0.86mL/g VSS）的增强，分别是空白组的3.8倍和5.1倍.

Role of free nitrous acid on waste activated sludge bio-electrolysis and key microflora shift

It is a major bottleneck as efficient energy recovery from waste activated sludge (WAS) often require long treatment time during traditional anaerobic fermentation. In order to further enhance the resource utilization efficiency and shorten the treatment time, bio-eletrolysis, i.e., microbial electrolysis cells (MECs), assisted with free nitrous acid (FNA) was employed for WAS treatment in this study. The performance of current and hydrogen generation during bio-eletrolysis from FNA-treated WAS was compared with that obtained from un-pretreated sludge. FNA significantly boosted the hydrolysis and acidification of WAS in MECs, in detail, the concentrations of soluble carbohydrates, proteins and volatile fatty acids (VFAs) were much higher than that of un-pretreated sludge. The utilization efficiency of VFAs was higher than 97% in the MEC-FNA test with the increase of current (1.9mA) and hydrogen yield (0.86mL/g VSS), which were 3.8 and 5.1 folds higher than that in the control. What's more, pyrosequencing revealed that the abundance of anaerobic fermentation bacteria, electrochemically active bacteria and nitrate-reducing bacteria were notably enhanced.