基于MLST鄱阳湖微囊藻的遗传多样性及其系统发育

为了研究鄱阳湖微囊藻的遗传多样性及其系统发育关系，基于7个管家基因（ftsZ、glnA、gltX、gyrB、pgi、recA和tpi），建立了一个多位点序列分型（MLST）方法.对来自鄱阳湖的20株微囊藻分离株进行MLST研究，并构建本地微囊藻MLST基因库.结果表明：这20株藻株具有20种独特的序列型（STs），揭示了微囊藻高水平的遗传多样性（H=0.986）.基于7个MLST位点串联序列的系统发育分析显示，微囊藻藻株序列型可分为5个不同的组.与之前日本湖泊研究的237个STs共建的系统发育树比对表明，本研究的STs形成了独立的分枝.该结果说明不同地区的微囊藻是相对稳定的，因此可以用MLST进行明确的表征.

Genetic diversity and phylogenetic analysis of Microcystis in Poyang Lake based on MLST

Using the multilocus sequence typing (MLST) based on seven housekeeping loci (ftsZ, glnA, gltX, gyrB, pgi, recA and tpi), twenty Microcystis strain isolated from Lake Poyang, the largest freshwater Lake in China, were examined to study their genetic diversity and phylogenetic relationship. The MLST revealed the 20 unique sequence types (STs) for these 20 Microcystis strains and their higher genetic diversity with H value as 0.986. The phylogenetic analysis based on the seven gene sequences showed that Microcystis strains were divided into five different groups, Phylogenetic tree based on the STs from our research and Japanese lakes revealed that our STs formed the independent branch, indicating that microcysts from different areas is relatively stable. So it can be clearly characterized by MLST.