热反硝化地芽孢杆菌水解催化剩余污泥提取蛋白质

为实现剩余污泥无害化处理以及资源化利用，采用从高温堆肥基质中筛选出的可产蛋白酶、淀粉酶等胞外酶的嗜热菌—热反硝化地芽孢杆菌对污泥氮源进行提取，分析反应时间、污泥含固率以及嗜热菌粗酶液接种量对蛋白质提取的影响，并确定最佳反应条件.结果表明：水解过程中各因素对蛋白质回收率作用大小为：接种量>时间>含固率，得出最优工艺条件为：pH=7、T=60℃、t=8h、污泥含固率为4%以及嗜热菌接种量为20%，在此条件下污泥蛋白质提取率达到32.05%，氨基酸含量为359.6mg/L，多肽含量为1060.1mg/L，且污泥上清液粒径随着反应的进行逐渐减小.

The extraction of protein from surplus sludge catalyzed by thermophilic Bacillus terrestris hydrolysis

For optimizing the processing conditions to realizing the harmless treatment and resource utilization of excess sludge, the thermophilic bacteria (Bacillus terrestris), which can produce extracellular enzymes such as protease and amylase, were selected from the high-temperature compost substrate and used to extract sludge nitrogen source in this study. Then effects of reaction time, sludge solid content and inoculum amount of crude enzyme solution of thermophilic bacteria on protein extraction were evaluated to determine the optimum reaction conditions. The results show that effects of various factors on protein recovery during hydrolysis were in the order of inoculation amount > time> solid content; and the optimal processing conditions included pH=7, T=60℃, t=8h, and solid content of 4% under which we observed thermophilic bacteria inoculation rate of 20%, protein extraction rate of 32.05%, amino acid content of 359.6mg/L, and polypeptide content of 1060.1mg/L, and that the sludge supernatant particle size decreased gradually with the reaction.