室内环境嗜松青霉的MVOCs释放特征及影响因素

以嗜松青霉为例,用顶空-固相微萃取-气相色谱-质谱联用仪(HS-SPME-GC-MS)采集分析其代谢挥发性有机物(MVOCs),考察了生长时间、温度、光照、氧含量(通气流量)对MVOCs释放特征的作用,探讨了其对室内VOCs的源贡献。结果表明,共发现6类14种MVOCs,包括浓度较高的乙醇、丙酮、乙酸、乙酸乙酯、异戊醇、苯甲醚,浓度较低的异丁醇、2-甲基丁醇、糠醇、甲苯、间二甲苯、1-辛烯-3-醇、3-辛醇和柠檬烯。氧含量和生长时间对MVOCs影响最明显,氧含量主要影响乙醇和丙酮的竞争代谢,乙酸、乙酸乙酯也在缺氧时产量更多;嗜松青霉在不同的生长阶段的代谢产物种类和产量均不同,总MVOCs量在接种第4~6d达到最大值,除乙酸、柠檬烯外,其他物质均受生长时间的影响;在25℃时能产生最多的MVOCs量,在35℃时产生的MVOCs最少,不同温度下各MVOCs组分也不同;光照对嗜松青霉的MVOCs代谢影响不明显。在有氧有光的3种实验温度下,总MVOCs排放强度在5031~7650ng/(m²×d)之间,各物质室内浓度贡献区间为0.0256~444.0380ng/m³。

Characteristics and influencing factors of microbial volatile organic compounds from a common indoor mold

Penicillium pinophilum was used as an example in this study and headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to collect and analyze microbial volatile organic compounds (MVOCs). The effects of the growth time, the temperature, the light and the oxygen content on the characteristics of MVOCs were investigated, and each contribution to indoor VOCs was discussed. A total of 6categories and 14 MVOCs were detected, including higher-concentration MVOCs such as ethanol, acetone, acetic acid, ethyl acetate, isoamyl alcohol and anisole, and lower-concentration MVOCs such as isobutanol, 2-methylbutanol, 2-Furanmethanol, toluene, m-xylene, 1-octene-3-ol, 3-octanol and limonene. The most obvious effects on MVOCs emission were the oxygen content and the growth time, which mainly affected the competitive metabolism of ethanol and acetone. The amounts of acetic acid and ethyl acetate were higher when the oxygen content was lower. There were considerable discrepancies of the types and the quantities of metabolites of Penicillium pinophilum along the growth time. The total amount of MVOCs reached maximum on the 4th~6th day of inoculation. Except acetic acid and limonene, other substances were obviously affected by the growth time. The maximum amount of MVOCs was produced at 25℃, and the minimum was at 35℃. The components of MVOCs were influenced by temperature. The light intensity had no obvious effect on the metabolism of Penicillium pinophilum. Under three experimental temperatures with certain oxygen content and light, the emission intensity of total MVOCs was between 5031and 7650ng/(m²×d), and the contribution of MVOCs concentrations to indoor air ranged from 0.0256 to 444.0380ng/m³.