彗星实验检测渤海主要入海河流遗传毒性

利用彗星实验检测渤海区主要入海河流遗传毒性.以虾虎鱼为受试生物,暂养在河口水样中,染毒48h,取外周血细胞,运用彗星实验检测外周血细胞内DNA损伤程度,以尾相(TM)作为DNA损伤程度指标,并据此评估入海河流邻近海域遗传毒性风险.实验结果表明,入海河流中的特征污染物可导致虾虎鱼外周血细胞的DNA损伤,且损伤程度可以通过彗星实验定量分析,同时该试验方法操作简便、快速、灵敏度高,能够反映出多种污染因子的综合致毒能力.因此,通过彗星实验建立实验室检测入海河流遗传毒性方法具有可行行和创新性.

Evaluation and detection of genotoxicity in main estuaries of Bohai Rim by comet assay

This paper reports an approach to detect genotoxicity of characteristic pollutants using Comet Assay in the Bohai estuaries. Gobies, a tested organism were temporary reared in estuary and exposed to toxicant 48h. Collecting the Gobies’ peripheral blood cells is to detect its tail moment (TM), which is an indicator of DNA damage condition. And thus assess the risk of estuary genotoxicity. The simple, fast and high-sensitive method allows analyzing the damage condition by using Comet Assay quantitatively and indicates the characteristic pollutants of estuary may cause DNA damage of Gobies’ peripheral blood cell. Thus, the base-on-lab method of detecting estuary pollutants by Comet Assay is feasible and innovative.