机动车排出物对人体健康危害的研究

运用基因、ＤＮＡ、染色体，细胞和整个动物为终点的远期危害测试系统，对各类机动车排出尾气的有机提取物的致突变性及潜在致癌性进行了评估，同时运用流行病学方法对接触人群进行调查，结果表明：（１）柴油车，助动车的颗粒有机提取物包含１００多种化学物，（２）Ａｍｅｓ、ＵＤＳ微核及ＳＨＥ细胞恶性转化试验为阳性结果，具有突变性及致癌性。（３）它们能使人胚细胞形成转化灶并发现其中的ｃ－ｍｙｃ，ｐ２１及ｂｃｌ－２表达

Harmful Effect of Vehicle Exhaust on Human Health

Using a series of assay standing for difference gentoxity end points, such as gene, DNA, chromosome, cells and animals to assess the risk of toxicity and harmful effect of various vehicle exhaust particle extract, especially the mutagenicity and potential carcinogenicity. The results as follows: (1) There are more than 100 composites in the organic extract of exhaust particles, some of them are mutagenical or have potential carcinogenicity; (2) They all showed positive effects in Ames assay, UDS, micronucleus assay and SHE cell malignantly transformed assay and strong mutagenicity; (3) Those extracts could make the KMB-13 cells to form transformed foci, in which the oncogene protein expression, such as c-myc, p21, bcl-2 were higher than ever; (4) after inhaling the exhaust, animal's pulmonary and immune function were be damaged; (5) The symptoms of respiratory tract and the risk of COPD (chronic obstructive pulmonary diseases) are rised in the occupational exposed workers.