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一种耐酚菌种及其固定化细胞降解含酚废水性能的比较研究
引用本文:孙 艳,李 京,谭立扬.一种耐酚菌种及其固定化细胞降解含酚废水性能的比较研究[J].环境科学研究,1999,12(1):1-4.
作者姓名:孙 艳  李 京  谭立扬
作者单位:1.北京大学生命科学学院蛋白质工程国家实验室, 北京 100871
摘    要:通过对土壤及污水的采样、分离、纯化,得到了一种降解苯酚的菌种,经过一系列驯化实验,使其苯酚耐受能力达915 mg/L。在此基础上,利用正交实验,确定了该菌种固定化细胞制备的最优操作条件。之后,对游离细胞和固定化细胞的降解苯酚过程进行了动力学分析与比较。结果表明,2种类型细胞的降解苯酚过程均符合Monod模型,并且固定化细胞的降解效果明显高于游离细胞。 

关 键 词:苯酚    固定化细胞    正交实验    Monod模型
收稿时间:1998/3/30 0:00:00

Study and Comparation of a Kind of Microorganism and Its Immobilized Cells for Phenol Degradation
SUN Yan,LI Jing and TAN Li-yang.Study and Comparation of a Kind of Microorganism and Its Immobilized Cells for Phenol Degradation[J].Research of Environmental Sciences,1999,12(1):1-4.
Authors:SUN Yan  LI Jing and TAN Li-yang
Institution:1.National Laboratory of Protein Engineerign, College of Life Sciences, Peking University, Beijing 1008712.Department of Chemical and Environmental Engineering, Beijing Polytechnic University, Beijing 100022
Abstract:In this work, a strain of bacteria isolated form sewage and soil nearby coking plant has been reported. The strain uses phenol as sole carbon source and degrades phenol up to the concentration of 915mg/L. Optimal preparation condition of the strain immobilization is carried out by the cross test experiments.Going further into the kinetic study of biodegradation for both free and immobilized cells proceeds in the simulated wastewater. Results obtained from degradation experiments agree fairly well with the Monod model. The final experimental results show that the degradation efficiency of immobilized cells is apparently superior to that of free cells.
Keywords:Phenol  Immobilized cells  Cross test  Monod model
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