利用专性杀菌剂进行煤矸石山酸化污染原位控制试验

酸性煤矸石山中硫化物的微生物氧化，加剧了煤矸石山酸化、自燃和爆炸等环境污染、灾害. 通过分离纯化氧化亚铁硫杆菌，并添加不同剂量的杀菌剂，分析了Fe2+氧化抑制率、杀菌剂的使用效果及最佳使用浓度. 结果显示:试验用杀菌剂SLS和SBZ能有效地抑制煤矸石山的酸化. ρ（SLS）为10 mg/L时Fe2+的氧化抑制率达到75.69%；ρ（SBZ）为30 mg/L时Fe2+的氧化抑制率达到75.89%. 表明杀菌剂的使用可以促进煤矸石山的酸化污染控制、绿化和生态重建. 

Selective Bactericides for at-Source Pollution Control of Acid Coal Waste Piles

The microorganism oxidation of sulphide minerals （FeS₂） in coal mine wastes results in some environmental problems and disasters such as acid mine drainage （AMD）, spontaneous combustion, and deflagration. The inhibitation of Fe2+ oxidation was analyzed by adding different dose of bactericides into the solution of purified Thiobacillus ferroxidans. The effects of two bactericides, sodium lauryl sulphate （SLS） and sodium benzoate （SBZ） in inhibiting the activity of Thiobacillus ferroxidans in coal waste piles were researched. The lab experimentshowed that both bactericides （SLS and SBZ） are effective in inhibiting the activity of Thiobacillus ferroxidans. The inhibition rate of controlling Fe2+ oxidation is 75.69% with SLS at the concentration of 10　mg/L, and that of SBZ is 75.89% at the concentration of 30 mg/L.Bactericide technology could control the pollution and promote the re-vegetation of coal mine wastes.