| 聚乙烯醇-硼酸固定化方法的改进 |
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| 引用本文： | 李花子,王建龙,文湘华,施汉昌.聚乙烯醇-硼酸固定化方法的改进[J].环境科学研究,2002,15(5):25-27. |
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| 作者姓名： | 李花子 王建龙 文湘华 施汉昌 |
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| 作者单位： | 清华大学,环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084 |
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| 基金项目： | 86 3计划资助项目( 86 3- 818-Q - 0 8) |
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| 摘 要： | 在固定化细胞技术中，常采用聚乙烯醇作为包埋介质，但聚乙烯醇固定化颗粒水溶膨胀性较大，易碎。笔者采用延时包埋法与加入化学药剂法对其进行了改进。结果表明，用这两种方法制得的聚乙烯醇固定颗粒的水溶膨胀性大大减少，不易破碎，电镜观察发现，改进后的聚乙烯醇凝胶网状结构明显优于未经过改进的聚乙烯醇凝胶的网状结构。
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| 关 键 词： | 聚乙烯醇 硼酸 固定代细胞技术 包埋法 水处理 化学药剂法 |
| 文章编号： | 1001-6929(2002)05-0025-03 |
| 收稿时间： | 2002/1/10 0:00:00 |
Improvement of PVA-H3BO3 Immobilization Method |
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| LI Hua-zi,WANG Jian-long,WEN Xiang-hua and SHI Han-chang.Improvement of PVA-H3BO3 Immobilization Method[J].Research of Environmental Sciences,2002,15(5):25-27. |
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| Authors: | LI Hua-zi WANG Jian-long WEN Xiang-hua and SHI Han-chang |
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| Institution: | State Key Joint Laboratory of Environment Simulation and Pollution Control,Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China;State Key Joint Laboratory of Environment Simulation and Pollution Control,Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China;State Key Joint Laboratory of Environment Simulation and Pollution Control,Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China;State Key Joint Laboratory of Environment Simulation and Pollution Control,Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China |
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| Abstract: | The PVA-H\-3BO\-3 method is often used for cell immobilization.But the gel beads are very easy to swell up and break up in water.Time\|extended immobilization method and the method of addition of chemical medicine were studied and proved to be good in reducing the gel beads swelling and improving its resistance to fragility.The electron microscopic observations show that the gel bead made at the improved method showed better gel mesh structure. |
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| Keywords: | PVA-H\-3BO\-3 method immobilization improvement |
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