一株异养硝化-好氧反硝化菌的脱氮性能研究

选用四因素三水平L₉(34)正交试验表设计实验，通过测定对NO₃－-N(硝酸盐氮)和TIN的去除能力，研究碳源、碳氮比(ρ(COD_Cr)/ρ(N))、溶解氧含量(ρ(DO))以及pH 4种不同因素对一株恶臭假单胞菌好氧反硝化性能的影响. 结果表明，该菌株对NO₃－-N的最大还原率可达100%；对NO₃－-N还原率影响最大的因素为ρ(COD_Cr)/ρ(N)，其次为ρ(DO)，碳源和pH；对应的最优条件:碳源为柠檬酸三钠，ρ(COD_Cr)/ρ(N)15，转速为60 r/min，pH为6.5.对TIN去除率影响最大的因素为ρ(COD_Cr)/ρ(N)，其次为碳源，ρ(DO)和pH； 对应的最优条件:碳源为柠檬酸三钠，ρ(COD_Cr)/ρ(N)15，转速为100 r/min，pH为6.5. 同时又对该菌株的异养硝化能力进行了测定发现，该菌株自身可实现同步硝化反硝化，其对氨氮的去除率可达60.91%，即该菌株可以独立完成生物脱氮的全部过程. 

Study on Denitrification Characteristics of a Heterotrophic Nitrification-Aerobic Denitrifier

The orthogonal experiment of four factors and three levels L₉ (34) was designed to research the best aerobic denitrification conditions for strains Pseudomonas putida. The four factors are carbon source, ρ(COD_Cr)/ρ(N), ρ(DO) and pH. The result shows that the best NO₃－-N reduction rate is 100%, and the most influencing factor on NO₃－-N reduction rate is ρ(COD_Cr)/ρ(N), and then is ρ(DO), carbon source and pH. The best condition is carbon source is sodium citrate, ρ(COD_Cr)/ρ(N) is 15, n60　r/min and pH6.5; The most influencing factor on TIN removal rate is ρ(COD_Cr)/ρ(N), and then is carbon source, ρ(DO) and pH. The best condition is carbon source is sodium citrate, ρ(COD_Cr)/ρ(N) is 15, n100　r/min and pH6.5. Meanwhile heterotrophic nitrification characteristics of this strainwee measured. Result is that this strain is able to realize SND, and NH₄＋-N removal rate is 60.91%. It shows that this strain can perform the whole process ofbacteria denitrification independently.