16S rDNA克隆文库方法分析好氧颗粒污泥细菌组成

采用构建16S rDNA克隆文库方法对好氧颗粒污泥的细菌种群多样性进行研究. 随机测定了82个克隆子序列(700 bp)，Blast比对结果表明，好氧颗粒污泥中微生物群落具有高度多样性，可分为7个主要类群，其中，β变形菌(β-Proteobacteria)类群和鞘脂杆菌(Sphingobacteria)类群在文库中所占比例最大，分别为34.16%和30.50%；其次是Candidate division TM7类群、黄杆菌(Flavobacteria)类群和γ变形菌(γ-Proteobacteria)类群，分别为9.76%,7.32%和7.32%；放线菌(Actinobacteria)类群和α变形菌(α-Proteobacteria)类群所占比例相对较小，分别为4.88%和1.22%. 序列分析结果表明，好氧颗粒污泥中不仅含有对好氧颗粒污泥形成和稳定运行具有重要作用的食酸菌属(Acidovorax)细菌、假单胞菌(Pseudomonas)等细菌，还含有对CODCr和氨氮具有很好去除能力的Micropruina glycogenica，丛毛单胞菌科(Comamonadaceae)等细菌. 

Analysis of Bacterial Composition of Aerobic Granular Sludge with 16S rDNA Clone Library

The bacterial diversity of an aerobic granular sludge was studied by the construction of a 16SrDNA clone library. 82 clones were randomly selected from the library. The corresponding partial 16S rDNA gene (ca. 700 bp) was sequenced and blasted. The results indicated that the bacterial community in the aerobic granular sludge was highly diverse, and could be divided into seven major groups. The largest fractions were β-Proteobacteria and Sphingobacteria, which accounted for 34.16% and 30.50% of the total clones, respectively. The portions of Candidate division TM7, Flavobacteria andγ-Proteobacteria were 9.76%, 7.32%, and 7.32%, respectively. The relatively lower portions were 4.88% Actinobacteria and 1.22%α-Proteobacteria. The result of sequence analysis also shows that there are not only bacteria important for the forming and stable operation of aerobic granular sludge (for example, Acidovorax, Pseudomonas), but also bacteria beneficial to the removal of COD_Cr andammonia-nitrogen (for example, Micropruina glycogenica, Comamonadaceae) in the aerobic granular sludge.