一株甲基对硫磷高效降解菌的鉴定及特性研究

从长期施用甲基对硫磷(MP)的污染土壤中分离到一株能以甲基对硫磷为唯一碳源和氮源生长的新型降解菌HS-D38,并利用该菌既降解MP.结果表明,该菌既在降解MP的同时,可对中间产物对硝基苯酚(PNP)进行降解.该菌既能利用苯胺类物质作为唯一的氮源生长,又能利用对苯二酚作为唯一的碳源生长.经SDS或吖啶橙消除质粒后,HS-D38降解MP和PNP的能力丧失. 表明该菌降解酶可能由质粒DNA编码.对该菌16S rDNA 进行PCR扩增、测序,运用BLAST检索分析并构建了系统进化树.结合生理生化鉴定结果,HS-D38被鉴定为铜绿假单胞菌(Pseudomonas aeruginosa).

Study on Isolation and Characteristic Identification of a Novel High-Efficiency Degrading Strain of Methyl Parathion

A bacterium strain HS-D38 capable of degrading methyl parathion(MP) was isolated from the soil in which organophosphorus pesticides had been used for a long period.This strain can use MP as the sole carbon and nitrogen sources.It was used to degrade MP and it was indicated that the strain could also degrade p-nitrophenol(PNP) produced in the degradation of MP.The strain of HSD38 can use phenylamine as the sole nitrogen source and hydroquinone as the sole carbon source.The plasmid-cured strain could not degrade MP and PNP,which indicated that the enzyme of degradation was involved in plasmid.The characteristics of HS-D38 strain were investigated including cell morphology,biochemical characteristics,16S rDNA amplification,sequencing,and BLAST analysis.The phylogenetic tree of HS-D38 was constructed.All these results indicated that HS-D38 belonged to Pseudomonas aeruginosa.