Carrousel 2000 氧化沟工艺中前置缺氧池与厌氧池的微生物多样性分析

2005年4—8月采集了Carrousel 2000氧化沟工艺调试过程中缺氧池和厌氧池中的活性污泥样品，直接提取样品的基因组DNA并纯化，对细菌16S rDNA的V₃高变区进行PCR扩增和DGGE分离，通过比较DGGE指纹的相似性来研究氧化沟的调试过程中微生物的变化情况. 结果表明，缺氧池中戴斯系数(Cs)最大值:4月为68.2%，5—6月为57.7%，8月为83.6%；厌氧池中Cs最大值:4月为64.8%，5—6月为62.7%，8月为71.5%. 缺氧池与厌氧池的微生物多样性均表现为先减少后增加的趋势. 两池间的微生物种群相似性逐渐降低，启动初期(4月)Cs最大值为80.5%；调试中期(5—6月)Cs最大值为60.8%；调试后期(8月)Cs最大值为59.3%. 好氧接种污泥在缺氧池和厌氧池中的驯化期为4个月左右. 

Analysis of Microbial Diversity in Pre-Anoxic and Anaerobic Reactors in the Carrousel 2000 Oxidation Ditch

Activated sludge in pre-anoxic and anaerobic reactors in the starting-up of Carrousel 2000 oxidation ditch was sampled from April to August in 2005, and their genomic DNA was extracted directly and purified. Then the 16S rDNA genes (V₃ region) were amplified and gradient gel electrophoresis denatured (DGGE). Microbial diversity was studied through comparing the DGGE fingerprints. The results showed that the maximum coefficient similarity is 68.2% in April, 57.7% in May to June and 83.6% in August in anoxic reactor, and it is from 64.8% to 62.7% and then to 71.5% in anaerobic reactor at the similar time. The microbial community in the two reactors shows the trend of increasing at first and then decreasing. The similarity between the two reactors becomes decreasing, the maximum coefficient similarity in the three phrases is 80.5% (starting-up phase), 60.8% (middle adjustment phase), and 59.3% (later adjustment phase). In conclusion, the domestication of microbial community in aerobic activated sludge in Carrousel 2000 oxidation ditch was about four months.