| E2诱导的鲫鱼(Carassius auratus)幼鱼血清中卵黄蛋白原和钙含量相关性研究 |
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| 引用本文： | 赵 兵,刘征涛,徐章法,方征,王琬华.E2诱导的鲫鱼(Carassius auratus)幼鱼血清中卵黄蛋白原和钙含量相关性研究[J].环境科学研究,2006,19(2):23-26. |
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| 作者姓名： | 赵 兵 刘征涛 徐章法 方征 王琬华 |
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| 作者单位： | 1.中国环境科学研究院,国家环境保护化学品生态效应与风险评估重点实验室,北京,100012;华东师范大学,环境科学系,上海,200061 |
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| 摘 要： | 用酶联免疫吸附法(ELISA)对E2诱导的鲫鱼幼鱼血清中卵黄蛋白原(VTG)进行了测定,并用原子吸收分光光度法测定了血清中的钙含量.结果表明:鲫鱼幼鱼血清中VTG和钙含量与E2的暴露剂量均有很好的剂量-效应关系.对血清中VTG和钙含量进行回归分析,发现二者具有较好的相关性,R2=0.928 7.因此,用原子吸收法测定血清中的钙含量从一定程度上可以替代ELISA法间接确定血清中的VTG含量,并对化学物质的雌激素效应进行了评价.
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| 关 键 词： | 鲫鱼幼鱼 卵黄蛋白原 酶联免疫吸附 原子吸收 相关性 |
| 文章编号： | 1001-6929（2006）02-0023-04 |
| 收稿时间： | 2005-05-15 |
| 修稿时间： | 2005-05-15 |
Studies on the Correlation between Serum VTG and Serum Calcium of Juvenile Carassius auratus after Exposure to E2 |
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| ZHAO Bing,LIU Zheng-tao,XU Zhang-f,FANG Zheng and WANG Wan-hua.Studies on the Correlation between Serum VTG and Serum Calcium of Juvenile Carassius auratus after Exposure to E2[J].Research of Environmental Sciences,2006,19(2):23-26. |
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| Authors: | ZHAO Bing LIU Zheng-tao XU Zhang-f FANG Zheng and WANG Wan-hua |
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| Institution: | 1. State Environmental Protection Key Laboratory for Ecological Effect and Risk Assessment of Chemicals, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; 2.Department of Environment, East China Normal University, Shanghai 200061, China |
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| Abstract: | Serum vitellogenin(VTG) was determined after exposure of juvenile Carassius auratus to E_2 using enzyme-linked immunosorbent assay(ELISA),and serum calcium was determined by flame atomic absorption spectroscopy.The results demonstrated that there was good dose-related response between the concentration of E_2 and serum VTG as well as serum calcium of juvenile Carassius auratus.Significant linear correlation between serum VTG and serum calcium for juvenile Carassius auratus(R~2=(0.928 7)) was also founded.So,serum calcium could act as a surrogate measure for ELISA to indirectly determine serum VTG to some extent and evaluate the estrogenicity of some chemicals. |
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| Keywords: | juvenile Carassius auratus VTG ELISA atomic absorption spectroscopy correlation |
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