耐高温解无机磷菌解磷条件的优化

为提高堆肥中添加难溶性磷的转化效率，对筛选于高温堆肥中的耐高温解无机磷菌株(BL161)解磷条件进行优化研究. 利用正交设计表L₂₅(56)选择培养时间，温度，pH，磷矿粉添加量和菌剂接种比例作为参考因素，对该菌株进行发酵培养. 结果表明:该菌株的最佳解无机磷条件是培养基pH为4.5，磷矿粉添加量与接种量分别为9.0 g/L和7%，于38 ℃下培养25 d，解磷总量(274 mg/L)达到最大；对温度指标分析显示，该菌株的温度耐受范围较广，在34～50 ℃下均能进行解无机磷活动，38 ℃为最适解无机磷培养温度；该菌株的耐热性较高，在50 ℃下能够很好地定植和解无机磷，发酵液中ρ(磷矿粉)随着培养时间的增加呈上升趋势，并于培养的第25天达到209 mg/L；极差分析显示，发酵时间对解磷总量的影响最大(R_时间152.2)，其他各因素对解磷总量的影响大小依次为pH＞温度＞磷矿粉添加量＞接种量. 

Optimizing the Solution Condition of a Thermostable and Soluable Inorganic Phosphorus Microorganism

In order to increase the conversion efficiency of insoluble phosphorus in compost,a phosphorous dissolving condition was optimized for heat-resistant inorganic phosphorus strains(BL161) screened from high-temperature compost.Orthogonal design table L_(25)(5~6) was used to select time,temperature, pH,additive phosphorus quantity and proportion of inoculum as reference factors in BL161 fermentation.The results of the orthogonal design software analysis indicated that optimal inorganic phosphorus degradation c...