共基质对优势菌降解多环芳烃的作用研究

以萘为唯一碳源驯化长期被焦化废水污染的污泥，7周后，平板划线分离出两株株黄杆菌PCN1，PCN2及一株短杆菌BCN1；并利用静态反应曝气及生物摇床试验对其降解菲、芘的效能进行了试验，同时研究了共基质及无机离子对它们降解菲、芘效果的影响。结果发现，BCN1对菲、芘的降解效果最好，淘米水地分离出的三株菌解菲或芘均有明显的共代谢促进作用。苯酚、尿素、葡萄糖对两株黄杆菌降解菲有抑制作用。加入Fe^3 使淘米水在两株黄杆菌降解菲的反应体系中的共代谢促进作用减小，但却增加了短杆菌反应体系中淘米水的促进作用。

The Study on Effects of Co-Substrates on the Biodegradation of Polycyclic Aromatic Hydrocarbons by Preponderant Bacteria

Two Flavobacterium FCN1,FCN2,and a Brevibacterium BCN1 were isolated from the sludge contaminated by coke-plant water for a long time.Before isolation,the sludge was cultivated by naphthalene as sole carbon source for 7 weeks.The degradation tests of phenanthrene and pyrene by our isolates were carried out under still-reaction-bottle-aeration and shaking-aeration conditions.The co-metabolic characteristics of several co-substrates and inorganic ions on the biodegradation of phenanthrene and pyrene by our isolates were also studied.The results show that phenanthrene and pyrene can be degraded by the three isolates effectively,the BCN1 is the most effective and that such degradation process can be accelerated by rinsing-rice-water.The phenol,urea and glucose play inhibiting actions on the degradation of phenanthrene under the existence of FCN1 and FCN2.All co-substrates used have promoting effects on the degradation of phenanthrene by BCN1.The ferric ion can decrease the promoting effect of the rinsing-rice-water in our two Flavobacterium reaction systems,but can increase that of the rinsing-rice-water in the BNC1 reaction system.