基于电生理信息的两种桑科植物叶片细胞代谢能比较

植物的细胞代谢能可以表征植物的生理活性和源库状况,而植物的细胞代谢能可由细胞的生理电容、生理电阻和生理阻抗来表征。本研究以桑科植物构树(Broussonetia papyrifera)和桑树(Morus alba)为例研究了不同夹持力下生理电阻、生理阻抗和生理电容的特征。先依据吉布斯自由能方程和Nernst方程构建模型,再基于模型的各个参数联合计算植物叶片细胞代谢能。结果表明,基于电阻计算和基于阻抗计算所获得的自由能相差不大;桑树叶片细胞的自由能(8.858×10~(-12)J)与构树叶片细胞自由能(8.486×10~(-12)J)相近,表明两种植物在实验环境下的生理活性相差不大;桑树叶片细胞的自由能绝对极差(10.865×10~(-12)J)小于构树(19.684×10~(-12)J),表明前者的生理活性变异性小于后者,后者叶片之间的源库关系较前者灵活;构树叶片比有效厚度绝对极差(6.380×10~(-12)m)大于桑树(3.999×10~(-12)m),表明构树叶片形态多样性与功能多样性相适应。本研究方法可以在线、快速、无损地表征植物叶片细胞代谢能,并为研究植物的生理活性和源库状况提供了新的方法和思路。

Comparison of Metabolic Energy in Two Mulberry Species Based on Leaf Electrophysiological Properties

The cellular metabolic energy of plants can be used to characterize the physiological activity and source-sink status of plants, while the cellular metabolic energy of plants can be characterized by the physiological capacitance, physiological resistance and physiological impedance of the cells. This study took Broussonetia papyrifera and Morus alba as examples to study the characteristics of physiological resistance, physiological impedance and physiological capacitance under different clamping forces. The model is first constructed based on the Gibbs free energy equation and the Nernst equation, and then the plant leaf cell metabolic energy is jointly calculated based on the various parameters of the model. The results showed that the free energy obtained based on resistance calculation and impedance calculation is not much different; and the free energy of Morus alba leaf cells (8.858×10^-12 J) is similar to that of Broussonetia papyrifera leaf cells (8.486×10^-12 J), which indicates that the physiological activities of the two plants in the experimental environment are not much different; the range of free energy of Morus alba leaf cells (10.865×10^-12 J) is smaller than that of Broussonetia papyrifera (19.684×10^-12 J), indicating that the former has a smaller variation in physiological activity, and the source-sink relationship between the leaves of the latter is more flexible than the former; the effective thickness of Broussonetia papyrifera leaves is extremely poor (6.380×10^-12 m) than that of Morus alba (3.999×10^-12 m), which indicates that the morphological diversity is compatible with the functional diversity. This research method can characterize plant leaf cell metabolic energy online, quickly and non-destructively, and provides new methods and ideas for studying plant physiological activities and source-sink status.