3株细菌对土壤中芘和苯并芘的降解及其动力学

研究了3株多环芳烃(PAHs)高效降解菌对土壤中芘和苯并芘(BaP)的降解动态,用Michaelis-Menton和Monod动力学模型对结果进行拟合.结果表明,3株细菌对芘和BaP的降解率有显著性差异.芽孢杆菌(Bacillus sp.SB02)42 d对芘和BaP的降解率均最高.当土壤中芘和BaP的初始浓度为50　mg/kg时,芽孢杆菌(Bacillus sp. SB02)、动胶杆菌(Zoogloea sp. SB09)、黄杆菌(Flavobacterium sp. SB10)42 d对芘的降解率分别为42.69%、32.88%、25.07%, 对BaP的降解率分别为33.04%、25.39％、22.02%.3株细菌对芘和BaP的降解速率也存在显著性差异.芽孢杆菌(Bacillus sp., SB02)最快,1周可降解20.88％芘和12.6%的BaP,动胶杆菌(Zoogloea sp.SB09)次之,黄杆菌(Flavobacterium sp.SB10)降解速率最慢.

Degradation and kinetics of pyrene and benzo [a] pyrene by three bacteria in contaminated soil

Degradation kinetics of pyrene and benzoa]pyrene(BaP) in soil by three individual strains of bacteria were examined using the Michaelis-Menton and Monod dynamic models.There were significant differences occurred in the degradation rates of pyrene and BaP by the three individual strains.When the initial concentrations of pyrene or BaP was 50 mg/kg in soil,the degradation rates of pyrene by Bacillus sp.SB02,Zoogloea sp.SB09,and Flavobacterium sp.SB10 in 42 days were 42.69%,32.88% and 25.07% respectively for pyrene.Similarly,degradation rates of BaP by Bacillus sp.SB02,Zoogloea sp.SB09,and Flavobacterium sp.SB10 in 42 days were 33.04%,25.39% and 22.02% respectively for BaP.In addition,there also were significant differences occurred in the degradation rates per day of pyrene and BaP by the three individual strains.The most rapid degradation of pyrene and BaP by Bacillus sp.SB02 occurred in one week,and 20.88% of pyrene and 12.6% of BaP were degraded.However,Zoogloea sp.SB09 had a slower degradation,while Flavobacterium sp.SB10 had the slowest degradation.