生物因子控制反冲洗对BEAC滤池优势菌稳定性影响研究

针对用于生物增强活性炭工艺(BEAC)中的6株优势菌,利用生物因子研究反冲洗对优势菌群在活性炭表面稳定性的影响,确定适合的反冲洗方式及强度.中试试验表明,气冲强度对BEAC生物膜的影响作用要比水冲洗大,通过比较反冲洗前后优势菌群生物量,确定BEAC滤池启动期采用单独水反冲洗,稳定期采用气水联合反冲洗,优势菌群在活性炭表面的平均生物损失量为15.47%;利用生物量和生物活性的乘积(生物需氧量,BRP)确定最佳反冲洗气强度为8～10 L/(m~2·s),反冲洗前后的生物量和生物活性变化最小;扫描电镜和PCR-DGGE图片结果表明,启动期采用气水联合反冲洗导致优势菌数量减少,稳定期采用气水联合反冲洗可保持优势菌的稳定性,反冲洗前后优势菌在活性炭表面数量变化较小.

Effect of Backwashing on Dominant Microorganism Stabilization of BEAC Filter Controlled by the Biological Factors

Six kinds of dominant microorganism used for the bioenhancement activated carbon(BEAC) filter were investigated for the study of backwashing effect on microorganism stabilization. The pilot plant results showed the average biomass loss was 15.47% when only water backwashing invited during the start-up period and air-water scour backwashing invited during stabilization operation period. The optimal backwashing parameters were determined by the biological factor of biomass respiration potential(BRP). The optimal air flow rate for the BEAC filter was 8-10 L/(m²·s) under which conditions the biomass and biological activity of the dominant microorganism had the minimum variation before and after backwashing. The SEM and PCR-DGGE photos showed that the biomass of dominant microorganism after air-water scour backwashing decreased during start-up period and retained stability during stabilization operation period.