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| 应用T-RFLP技术分析滇池污染水体的细菌群落 | |
| 引用本文： | 史青,柏耀辉,李宗逊,冯传平,温东辉.应用T-RFLP技术分析滇池污染水体的细菌群落[J].环境科学,2011,32(6):1786-1792. |
| 作者姓名： | 史青 柏耀辉 李宗逊 冯传平 温东辉 |
| 作者单位： | 1. 中国地质大学(北京)水资源与环境学院,北京,100083 2. 北京大学环境科学学院,北京,100871 3. 云南昆明市环境科学研究院,昆明,650032 |
| 基金项目： | 国家水体污染控制与治理科技重大专项（2009ZX07102-002-01）；国家自然科学基金项目（51008003） |
| 摘 要： | 为确定重富营养化湖泊--滇池水体的主要细菌类型与种群结构,本研究运用末端限制性片段长度多态性(T-RFLP)技术分析了滇池水体9个样点的细菌群落特征及多样性.对各样本的T-RFLP谱图进行聚类分析,结果表明不同地理位置的污染物浓度差异可能是造成细菌群落差异的主要原因.研究同时结合克隆文库技术对各样本T-RFLP谱图中的... |
| 关 键 词： | 滇池 细菌群落 多样性 优势菌 T-RFLP技术 |
| 收稿时间： | 8/5/2010 12:00:00 AM |
| 修稿时间： | 2010/9/20 0:00:00 |
Analysis of Bacterial Community in the Polluted Water of Dianchi Lake by Using T-RFLP Technique |
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| SHI Qing,BAI Yao-hui,LI Zong-xun,FENG Chuan-ping and WEN Dong-hui.Analysis of Bacterial Community in the Polluted Water of Dianchi Lake by Using T-RFLP Technique[J].Chinese Journal of Environmental Science,2011,32(6):1786-1792. | |
| Authors: | SHI Qing BAI Yao-hui LI Zong-xun FENG Chuan-ping and WEN Dong-hui |
| Abstract: | In order to assess the dominant microbial communities in the polluted water of the Dianchi Lake, terminal restriction fragment length polymorphism (T-RFLP) technique was used to analyze the characteristics and diversity of bacterial communities in nine sites. Clustering analysis based on the T-RFLP profiles showed that the bacterial community structures varies considerably, which may be caused by the pollutant variation at different geographic locations. According to the further analysis of T-RFLP profiles with clone library technique, the dominant bacteria groups in nine sites represented by distinctive terminal restriction fragments (T-RFs) were identified as genera of Pseudomonas, Aphanizomenon and Rhodobacter. |
| Keywords: | Dianchi Lake analysis of bacterial community diversity dominant bacteria T-RFLP |
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