酵母Candida krusei对合成染料的脱色

通过筛选实验,从土壤中新分离到1株对活性艳红K-2BP具有明显脱色效果的酵母菌株Y-G-1,经鉴定为克鲁斯假丝酵母Candida krusei.该菌株对含活性艳红K-2BP起始浓度为200mg/L的培养基,最大脱色率为99%,达到最大脱色率的时间是12h.克鲁斯假丝酵母的最佳接种量应不低于5%(体积分数),培养基最适pH在4～9之间,氮源(NH₄)₂SO₄浓度不低于0.1%,相对应的碳源葡萄糖浓度不低于0.5%.对脱色机理的研究表明,该菌株对活性艳红K-2BP的去除属于降解脱色.此外,该菌株对另外9种染料(50mg/L)的脱色率在62%～96%之间.其中,对偶氮染料弱酸艳红B、活性黑KN-B和活性红M-3BE的脱色率都达到了90%以上,对三苯甲烷染料(酸性媒介漂蓝B)的脱色率达到了93%.表明克鲁斯假丝酵母在染料废水的处理上可能具有较好地应用潜能.

Decolorization of Synthetic Dyes with a New Isolated Yeast Candida krusei

A new yeast isolate Y-G-1, identified as Candida krusei,capable of degrading Reactive Brilliant Red K-2BP, was obtained from soil by screening experiments. This strain gave a maximal decolorization (99%) for Reactive Brilliant Red K-2BP (200mg/L) after incubation of 12 h. In order to get this decolorization effect, the optimal inoculation volume of Candida krusei was not less than 5%, the optimal pH of culture medium was within a range from 4 to 9, and the concentrations of (NH₄)₂SO₄ and glucose were not lower than 0.1% and 0.5%, respectively. The research results on the mechanism of decolorization show that Candida krusei could biodegrade Reactive Brilliant Red K-2BP. In addition, Candida krusei could also decolorize 62%96% of another 9 dyes (50mg/L). Specially for azo dyes, including Weak Acid Brilliant Red B, Reactive Black KN-B and Reactive Red M-3BE, their decolorization rates were over 90%, and for triphenylmethane dye,Mordant Blue B, it was up to 93%. All these results indicate that this strain have potential application in dye wastewater treatment.