| 青草沙水库蓄水期间细菌群落结构变化的初步研究 |
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| 引用本文： | 彭青,谢冰,袁琦,黄智婷,崔璐璐,王文婷.青草沙水库蓄水期间细菌群落结构变化的初步研究[J].环境科学,2012,33(10):3634-3640. |
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| 作者姓名： | 彭青 谢冰 袁琦 黄智婷 崔璐璐 王文婷 |
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| 作者单位： | 华东师范大学资源与环境科学学院,上海市城市化生态过程与生态恢复重点实验室,上海200062 |
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| 基金项目： | 上海市建委重大科研项目(建重2008-05) |
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| 摘 要： | 为调查青草沙水库蓄水及运行初期水质变化和微生物群落的关系,采用微生物培养计数和PCR-DGGE(变性梯度胶凝胶电泳)技术对水库水体中的细菌群落结构进行了研究.结果表明,长江来水进入水库后,氮磷浓度显著降低,水质明显改善;来水和水库中可培养微生物数量随季节变化,春夏季来水明显多于库内,秋冬季少于库内,悬浮于水中的微生物等有机体可通过沉降作用部分进入到底泥中,导致库尾底泥有机质含量增加;PCR-DGGE分析结果表明库中细菌群落结构呈现季节性变化,夏季微生物群落多样性丰度最高,秋季较低;水库运行初期水样和底泥的微生物群落结构聚类分析的相似度可以达到62%,这与来自水中微生物的沉降有关;来水及库中主要的优势微生物与α、β-Proteobacteria、Flavobacterium、Rheinheimera、Prochlorococcus、Synechococcus和海洋宏基因具有很高的相似性,预示着水库存在藻类规模生长和海水入侵的风险,研究结果可为水库的运行管理和深入研究提供参考.
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| 关 键 词： | 青草沙水库 PCR-DGGE 细菌群落结构 微生物计数 氮磷营养盐 |
| 收稿时间： | 2011/12/19 0:00:00 |
| 修稿时间： | 2012/3/19 0:00:00 |
Preliminary Study on the Changes of Bacterial Community Structure in Qingcaosha Reservoir During Water Storage Period |
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| PENG Qing,XIE Bing,YUAN Qi,HUANG Zhi-ting,CUI Lu-lu and WANG Wen-ting.Preliminary Study on the Changes of Bacterial Community Structure in Qingcaosha Reservoir During Water Storage Period[J].Chinese Journal of Environmental Science,2012,33(10):3634-3640. |
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| Authors: | PENG Qing XIE Bing YUAN Qi HUANG Zhi-ting CUI Lu-lu and WANG Wen-ting |
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| Institution: | Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Resource and Environment Science, East China Normal University, Shanghai 200062, China;Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Resource and Environment Science, East China Normal University, Shanghai 200062, China;Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Resource and Environment Science, East China Normal University, Shanghai 200062, China;Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Resource and Environment Science, East China Normal University, Shanghai 200062, China;Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Resource and Environment Science, East China Normal University, Shanghai 200062, China;Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Resource and Environment Science, East China Normal University, Shanghai 200062, China |
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| Abstract: | |
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| Keywords: | Qingcaosha Reservoir PCR-DGGE bacterial community structure microbial culture counts nitrogen and phosphorus nutrients |
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