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一组小麦秸秆好氧分解菌系的构建及组成多样性
引用本文:董玉玲,朱万斌,郭鹏,王小芬,张利莉,崔宗均.一组小麦秸秆好氧分解菌系的构建及组成多样性[J].环境科学,2010,31(1):249-254.
作者姓名:董玉玲  朱万斌  郭鹏  王小芬  张利莉  崔宗均
作者单位:中国农业大学农学与生物技术学院;塔里木大学新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室;中国农业大学生物质工程中心;
基金项目:“十一五”国家科技支撑计划重大项目(2006BAD07A01);;教育部高校博士点新教师基金项目(20070019055);;国家自然科学基金青年基金项目(30800672)
摘    要:采用"外淘汰法"在常温、好氧条件下构建了一组稳定、有效降解小麦秸秆的复合系.复合系分解能力的研究表明,在100mL改良CMC培养基(液面深度2 cm和直径9 cm)中,分解的前6 d复合系保持较高分解能力,减重率达到66.1%,6 d后复合系分解能力逐渐减弱,到第10 d时减重率达到77.0%.1.86 g秸秆各成分中,纤维素分解0.78 g,半纤维素分解0.16 g,木质素分解0.24 g.复合系组成多样性的研究表明,通过克隆文库构建和单菌株分离共确定出13个菌属的微生物,优势菌属有Hydrogenophaga、Pseudomonas、Bacteroides和Clostridium,占100个阳性克隆子的78%.系统发育关系表明,克隆文库和单菌分离技术分别所确定的微生物种类及亲缘关系存在一定的差异;Isolated 7(FJ439527)和Clone 86(EU834839)与假单胞菌属中的厦门藻(Pseudomonas xiamenensis)亲缘关系较近.

关 键 词:小麦秸秆  木质纤维素  好氧分解  复合系  多样性  
收稿时间:2009/2/28 0:00:00
修稿时间:5/8/2009 12:00:00 AM

Characteristics and Diversity of a Microbial Community Decomposing Wheat Straw Under Aerobic Conditions
DONG Yu-ling,ZHU Wan-bin,GUO Peng,WANG Xiao-fen,ZHANG Li-li and CUI Zong-jun.Characteristics and Diversity of a Microbial Community Decomposing Wheat Straw Under Aerobic Conditions[J].Chinese Journal of Environmental Science,2010,31(1):249-254.
Authors:DONG Yu-ling  ZHU Wan-bin  GUO Peng  WANG Xiao-fen  ZHANG Li-li and CUI Zong-jun
Institution:1.College of Agronomy and Biotechnology;China Agricultural University;Beijing 100193;China;2.Center of Biomass Engineering;3.Key Laboratory of Protect and Utilization of Biology Resources in Tarim Basin of Xinjiang Product and Construct;Tarim University;Alaer 843300;China
Abstract:A microbial community with the stable ability of effectively decomposing wheat stalks was constructed at room temperature under aerobic conditions by the method of restricted cultivation.The degradation ability of the microbial community shows that when performed in 100 mL improved CMC liquid medium,the depth and diamerer of the fluid level were 2 cm and 9 cm,it kept higher degradation efficiency with capability of decomposing straws of 66.1% during the initial six days.By the tenth day of decomposition,the...
Keywords:wheat straw  lignocellulose  aerobic degradation  microbial community  diversity  
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