中常温变化对PN-ANAMMOX联合工艺脱氮效果的影响

通过接种成熟的亚硝化膜和厌氧氨氧化污泥,研究了中常温变化对PN-ANAMMOX联合工艺脱氮速率的影响及微生物群落的变化.结果表明,常温下能够实现PN-ANAMMOX联合脱氮,并且脱氮速率达到0.5 kg·(m~3·d)~(-1).但是PN过程亚硝化速率下降,ANAMMOX菌活性未得到充分发挥,导致PN-ANAMMOX联合工艺脱氮速率远低于中温条件下的1.75kg·(m~3·d)~(-1),出水水质较差.温度的上升易导致NOB的快速生长,PN过程失稳,但是通过增加回流量可对NOB的活性进行有效地控制.QPCR分析结果进一步表明接种中温环境下的AOB和ANAMMOX微生物在常温条件下不利于生长,出现部分死亡;当恢复到中温的环境时,相应的功能微生物出现了快速地生长.因此,在PN-ANAMMOX联合工艺的运行过程中应尽可能地满足功能微生物适宜的温度.

Effect of Moderate and Room Temperature on Nitrogen Removal in PN-ANAMMOX Process

The effect of moderate and room temperature on nitrogen removal rate and microbial community was studied in PN-ANAMMOX process by inoculating mature nitrification membrane and ANAMMOX sludge. The results showed that the PN-ANAMMOX combined process was achieved at room temperature, and the nitrogen removal rate reached 0.5 kg·(m³·d)^-1. But the activity of ANAMMOX did not reach its maximum due to the decreased NO₂^--N production rate, resulting in far lower nitrogen removal rate of PN-ANAMMOX than that at moderate temperature which reached 1.75 kg·(m³·d)^-1, and the effluent had poor water quality. When the temperature rose, the NOB grew rapidly, the PN process became unstable, but the activity of NOB could be effectively controlled by increasing the return flow. The results of QPCR analysis showed that the inoculation of AOB and ANAMMOX was not conducive to the growth at room temperature, and part of the microorganisms died. When the environment was recovered to moderate temperature, the corresponding functional microorganisms appeared to grow rapidly. Therefore, suitable temperature should be provided for the microorganisms during the operation of the PN-ANAMMOX combined process.