基于高通量测序的ABR厌氧氨氧化反应器各隔室细菌群落特征分析

为明确厌氧折流板反应器(ABR)稳定运行厌氧氨氧化反应后各隔室微生物群落结构特征,本文采用Miseq高通量测序分析技术,对ABR厌氧氨氧化反应器5个隔室的微生物分布规律进行了研究,结果表明,ABR反应器中脱氮微生物多样性较为丰富,变形菌门(Proteobacteria)占11.66%~20.28%,浮霉菌门(Planctomycetes)占2.18%~7.94%,硝化螺旋菌门(Nitrospirae)占0.19%~6.30%.其中,在ABR反应器中变形菌门占据主导地位,主要包含Rhodoplanes、Dok59、Rubrivivax和Bdellovibrio等菌属,浮酶菌门次之,主要包含Candidatus brocadia和Candidatus kuenenia菌属.从第1~5隔室,污泥表观红色逐渐减退,趋向于灰黑色,Chao、ACE、Shannon、Simpson指数均表明微生物群落丰富度逐渐增加,且变形菌门微生物逐渐增加,而浮霉菌门微生物逐渐降低,这与基质的降解和功能微生物的富集规律相一致.

Characteristics of Microbial Community in Each Compartment of ABR ANAMMOX Reactor Based on High-throughput Sequencing

In order to investigate the characteristics of microbial community in each compartment of ABR anammox reactor, a five-compartment ABR reactor was used to analyze the microbial community by Miseq High-throughput Sequencing during the steady operational process. The results indicated that the denitrifying bacteria coexisted in the reactor, such as Proteobacteria, Planctomycete, and Nitrospirae bacteria, and the percentages of these three microbial populations in the sludge were 11.66%-20.28%, 2.18%-7.94% and 0.19%-6.30%, respectively. In addition, there were four dominant genera in the phylum Proteobacteria:Rhodoplanes, Dok59, Rubrivivax and Bdellovibrio. Furthermore, Candidatus brocadia and Candidatus kuenenia were the main genera in the phylum Planctomycete. The color of sludge in the five compartments, in turn, varied from red to black. In addition, the biodiversity index of Chao, ACE, Shannon and Simpson indicated that the richness and diversity of microbial community increased gradually, and at the same time, the relative abundance of Proteobacteria increased while that of Planctomycetes gradually decreased. The above conclusion was consistent with the laws of substrate degradation and enrichment of functional microorganisms.