无机碳源对栅藻LX1生长及油脂积累特性的影响

以二级出水为培养基,比较了对照组和补充碳源（Na2CO3、NaHCO3和CO2）组栅藻LX1的生长和油脂积累特性.培养14d,4组试验中栅藻LX1的生长及油脂积累特性均无显著差别,藻细胞生物量、油脂及三酰甘油酯产量分别为0.70～0.77、0.27～0.34和0.029～0.033 g.L-1,单位藻细胞油脂含量约为3...

Effect of Inorganic Carbon Supplement on the Growth and Lipid Accumulation Properties of Scenedesmus sp. LX1

With secondary effluent as growth medium, the growth and lipid accumulation properties of Scenedesmus sp. LX1 in the control and carbon supplement (Na₂CO₃, NaHCO₃ and CO₂) groups were compared. After 14 days of cultivation, there was no significant difference in growth and lipid accumulation of Scenedesmus sp. LX1 among the 4 groups. The algal biomass, lipid and triacylglycerols production were 0.70-0.77 g·L^-1, 0.27-0.34 g·L^-1 and 0.029-0.033 g·L^-1, respectively, and the lipid content per algal biomass was 35%-45%. The microalgal photosynthesis would increase the water's pH to about 9.2, which could induce CO₂ transferring into secondary effluent. In control group, the amount of CO₂ transferring from atmosphere into secondary effluent was 1.0 g·L^-1, occupying 97% of total fixed CO₂ by microalga. Calculation shows that, the CO₂ transferring from atmosphere into secondary effluent is enough to support microalgal growth and lipid accumulation. Therefore, to cultivate Scenedesmus sp. LX1 in secondary effluent, there is no need of carbon supplement.