1株好氧脱氮菌的筛选与脱氮特性研究

研究好氧反硝化菌的筛选、生物脱氮机制及代谢特征.采用极限稀释及平板划线法对好氧条件下能同步硝化/反硝化的细菌进行广泛筛选,分离到6株具有好氧脱氮效果的异养菌,其中YY-5菌具有高效的好氧脱氮能力.对该菌株好氧脱氮过程气相和液相中可能生成的气态产物以及硝态氮等形式的含氮产物变化进行定量检测分析,探讨脱氮过程氮素的去向及其...

Screening of the Aerobic Simultaneous Denitrobacteria and Its Denitrification Characteristics

To screen aerobic heterotrophic denitrifying bacteria from various samples and detect metabolites quantitatively during their denitrification process. The bacteria were screened by limit dilution and crossed separation. Nitrate and nitrite were determined using phenol disulfonic acid and N-(1-naphthyl)-ethylene diamine by ultraviolet spectrophotometry. The N₂ was detected by gas chromatography. Six aerobic heterotrophic bacteria were isolated and YY-5 was demonstrated to be the greatest denitrifer. The degradation rate of its hydroxylamine oxidoreductase can reached to 70.9%. Above all, there is a good balance between the removal of total nitrogen and production of N₂ in process. No N₂O was produced and no nitrate or nitrite were accumulated. The high ability of aerobic heterotrophic bacterium YY-5 for denitrification was screened, which showed higher homology with Acinetobacter genus through 16S rRNA blast.