| 水介质中C60纳米晶体颗粒与Cu2+对小鼠腹腔巨噬细胞的复合毒性研究 |
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| 引用本文： | 于叶,卞为林,张慧敏,张波,何义亮.水介质中C60纳米晶体颗粒与Cu2+对小鼠腹腔巨噬细胞的复合毒性研究[J].环境科学,2015,36(7):2713-2718. |
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| 作者姓名： | 于叶 卞为林 张慧敏 张波 何义亮 |
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| 作者单位： | 上海交通大学环境科学与工程学院,上海,200240 |
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| 基金项目： | 国家自然科学基金项目(21177084);国家水体污染控制与治理科技重大专项(2014ZX07206001) |
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| 摘 要： | 为考察C60纳米晶体颗粒和Cu2+复合体系的生物毒性,本文以小鼠腹腔巨噬细胞RAW264.7作为受试对象,研究了C60纳米晶体颗粒物(n C60)和Cu2+对RAW 264.7的复合毒性影响.结果表明,当单独暴露n C6024 h,n C60浓度为6.6 mg·L-1时,细胞活性降低了45%,n C60浓度为9.9 mg·L-1时,细胞活性降低了70%,表现出剂量-毒性效应和时间-毒性效应.水中出现Cu2+可以降低n C60的细胞毒性,当Cu2+浓度为2 mg·L-1,n C60浓度为6.6 mg·L-1时,细胞的活性降低至25%,当Cu2+浓度为5 mg·L-1,n C60浓度为9.9 mg·L-1时,细胞的活性下降至15%.研究表明,n C60表面能够吸附Cu2+,并且吸附符合Langmuir吸附等温模型.n C60纳米晶体颗粒表面对Cu2+的吸附,可能是n C60对细胞毒性降低的重要原因.
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| 关 键 词： | C60 小鼠腹腔巨噬细胞 Cu2 + 吸附 细胞毒性 |
| 收稿时间： | 2014/10/14 0:00:00 |
| 修稿时间： | 2015/1/25 0:00:00 |
Coupled Cytotoxicity of C60 Nano-Crystal Particle with Cu2+ to the Mouse Peritoneal Microphage RAW 264.7 |
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| YU Ye,BIAN Wei-lin,ZHANG Hui-min,ZHANG Bo and HE Yi-liang.Coupled Cytotoxicity of C60 Nano-Crystal Particle with Cu2+ to the Mouse Peritoneal Microphage RAW 264.7[J].Chinese Journal of Environmental Science,2015,36(7):2713-2718. |
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| Authors: | YU Ye BIAN Wei-lin ZHANG Hui-min ZHANG Bo and HE Yi-liang |
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| Institution: | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China |
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| Abstract: | This work evaluated the cytotoxicity effect of nC60 nano-crystal particle associated with Cu2+by using mouse peritoneal microphage RAW264.7 as the test cell line. The results showed that when exposed to nC60 of 6.6 mg ·L-1 and 9.9 mg ·L-1 for 24h, the cell viability decreased 45% and 70%, respectively, and had obvious time-response and dose-response. It was found that Cu2+ could reduce the toxicity of nC60 on RAW 264.7. The cell viability reduced to 25% when exposed to 6.6 mg ·L-1 nC60 with 2 mg ·L-1 Cu2+, and reduced to 15% when exposed to 9.9 mg ·L-1 nC60 with 5 mg ·L-1 Cu2+. The nC60 could adsorb Cu2+and the adsorption isotherm was fitted to the Langmuir adsorption isotherm. The adsorption of Cu2+ on the surface of nC60 may decrease the cytotoxicity nC60 on RAW 264.7. |
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| Keywords: | C60 mouse peritoneal microphage Cu2+ adsorption cytotoxicity |
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