微生物絮凝剂MBFTRJ21的絮凝机理

筛选得到的乳酸杆菌属(Lactobacillus)具有用量少絮凝效果好等优点.蒽酮反应、考玛斯亮蓝、Elson-Morgan反应表明,絮凝剂MBFTRJ21为粘多糖类高分子絮凝剂;ξ电位测定及氢键和离子键检验结果表明,絮凝剂和碱泥之间的作用力为氢键;絮凝剂的热处理和KIO₄处理表明,其活性成分为蛋白质和糖胺;絮凝过程中粒度分析表明,絮凝过程存在架桥作用.其絮凝机理为:絮凝剂和碱泥在絮凝剂的活性部位--糖胺中的氨基以氢键的形式结合,然后再经过架桥作用絮凝沉淀.

Flocculating Mechanism of Microbial Flocculant MBFTRJ21

The bioflocculant MBFTRJ21 for nature soda was exploited in our lab, and its flocculating mechanism was studied. The bioflocculant produced by Lactobacillus, has some advantages, such as less dosage, better flocculating effects, and etc. MBFTRJ21 is mainly composed of polysaccharide, which is determined by Anthrone reaction, Coomassie blue reaction and Elson-Morgan method. The acting force between bioflocculant and nature soda is hydrogen bond, which is determined by xi electric potential, hydrogen bond and electrovalent bond determination. The bioflocculant is treated by heat and KIO4, which indicate the active ingredient of bioflocculant is protein and osamine. Bridge occur during flocculation process, which is determined by particle size distribution. The results showed that the flocculating mechanism of MBFTRJ21 resulted from the combination of nature soda with MBFTRJ21 by hydrogen bond at the active position of glycosaminoglycan.