基于A2/O处理工艺的生活污水的成组生物毒性评价

为了更加准确评估城市生活污水的综合生物毒性以及处理工艺对污水毒性的削减状况,本研究通过发光菌急性毒性、遗传毒性、雌激素活性检测方法对A~2/O工艺处理前后污水的毒性进行评价,同时通过斑马鱼暴露实验分析污水和回用水对水生生物内分泌干扰效应的作用模式.结果表明,污水厂进水具有较强的急性毒性、遗传毒性以及雌激素活性,水质较差.经二级生物处理后,上述毒性显著性降低,污水厂出水水质提高.但污水出水雌二醇当量为1.89 ng·L-1,仍可能会对受纳水体的水生生物产生潜在危害.浓缩2.5倍的水样导致雄性斑马鱼肝脏的卵黄原蛋白基因(vtg1)和雌激素受体基因(esr1)的表达水平显著上升,由此可以得出污水可通过干扰目的基因的表达来调控水生生物的内分泌活动.而esr1基因在肝脏的表达被抑制说明污水可能具有抗雌激素作用,同时反映进行生物毒性效应分析时应从多个组织或器官考虑,以获得比较全面的信息.

Biological Toxicity Evaluation of Domestic Wastewater Based on A2/O Treatment Processes Using a Battery of Bioassays

In order to accurately evaluate the comprehensive ecotoxicity of domestic wastewater and the toxicity reduction efficiency of different treatment processes, standardized assays focusing on Vibrio fischeri, SOS/umu test and yeast estrogen screen were applied. Simultaneously, male zebrafish was used to address the mode of action of endocrine disrupting effects of wastewater and reclaim water on aquatic life. Results of this study indicated that the influent was severely polluted:highly acute toxicity, genotoxicity and estrogenicity were observed. After the secondary biological treatment, the above toxicities were significantly reduced, making the water quality improved. However, the estrogencity of secondary effluent was detected at 1.89 ng·L^-1, which still posed potential risk on the aquatic life in receiving water. In addition, the up-regulation of estrogen receptor (esr 1) and vitellogenin (vtg 1) in the livers of zebrafish was observed after exposure to 2.5 times concentrated wastewaters, which demonstrated that wastewater modulates hormonal activities of aquatic life by disturbing target genes expression. Moreover, the slight down-regulation of esr 1 was observed in the gonads. These observations reflect that the evaluation of biological toxic effect should be analyzed in different tissues or organs, so that more comprehensive information about the adverse effects of wastewaters on aquatic life can be obtained.