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嗜碱细菌复合碳源条件下对麦草木质素的降解
引用本文:张甲耀,龚利萍,罗宇煊,许威亚,凌颉.嗜碱细菌复合碳源条件下对麦草木质素的降解[J].环境科学,2002,23(1):70-73.
作者姓名:张甲耀  龚利萍  罗宇煊  许威亚  凌颉
作者单位:1. 武汉大学环境科学系,武汉,430072
2. 武汉大学分析测试中心
基金项目:国家自然科学基金资助课题(29677012)
摘    要:在碱性液体培养条件下(pH≈10.5), 研究复合碳源共代谢最佳综合条件下嗜碱性木质素降解细菌6号菌株产酶、降解能力及菌株的生长状况.结果显示,虫漆酶(Laccase)在培养的第4天酶活达到最高值2915.37U/L、锰依赖过氧化物酶(MnP)在培养的第8天酶活达到最高值1152.88U/L,培养10d麦草中木质素降解49.84%.同时通过扫描电镜分析探讨了6号菌株降解木质素的微观过程,证明了6号菌株优先降解木质素的特性和降解方式.

关 键 词:嗜碱细菌  复合碳源  木质素生物降解  扫描电镜
文章编号:0250-3301(2002)01-04-0070
收稿时间:2001/2/16 0:00:00
修稿时间:2001年2月16日

Biodegradation of Lignin in Wheat Straw by Alkaliphilic Ligninolytic Bacteria with Compounded Carbons
Zhang Jiayao,Gong Liping,Luo Yuxuan,Xu Weiya and Ling Ji.Biodegradation of Lignin in Wheat Straw by Alkaliphilic Ligninolytic Bacteria with Compounded Carbons[J].Chinese Journal of Environmental Science,2002,23(1):70-73.
Authors:Zhang Jiayao  Gong Liping  Luo Yuxuan  Xu Weiya and Ling Ji
Institution:Department of Environmental Science, Wuhan University, Wuhan 430072, China.
Abstract:The article researched enzymes production, the degradation rate of lignin and strain growth of alkaliphilic ligninolytic bacteria strain No. 6 in alkaline liquid medium (pH approximately 10.5) with compounded carbons. The results showed that the highest activities of laccase and MnP were 2915.37 U/L (4th d) and 1152.88 U/L (8th d), respectively, and the strain degraded 49.84% lignin of wheat straw during ten days cultivation. The changes of ultrastructure of wheat straw caused by strain No. 6 attack were investigated with SEM (scan electron microscopy).
Keywords:alkaliphilic bacteria  compounded carbon  lignin biodegradation  SEM
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