接种白腐菌堆肥修复五氯酚污染的土壤

采用4组堆肥装置A、B、C、D,分别往五氯酚污染土壤中不加外来菌、加入固定化黄孢原毛平革菌(Phanerochaete chrysosporium)、加入游离黄孢原毛平革菌及暴露在空气中不做任何处理.对其进行对比研究,探讨了利用堆肥的方法对五氯酚污染土壤进行生物修复的可行性.结果说明,添加黄孢原毛平革菌进行堆肥修复其效果要好于不加菌的效果,经过60d的堆肥,PCP基本得到降解,降解率都达到了94%以上,其中固定化菌又优于游离菌,固定化菌在第9d对PCP的降解率就达到了50%.此外,通过分析种子发芽率,有机质,微生物量碳等多项指标可以看出各组堆肥的腐熟情况都比较理想,为黄孢原毛平革菌降解五氯酚的过程提供了良好的外部环境,使土壤修复与堆肥能较好地结合起来.

Remediation of pentachlorophenol-contaminated soil by composting with inoculation of white rot fungi

In the experiment four parallel beakers A,B,C,D were adopted,among which A was without any inoculum,B was added with the inocula of immobilized Phanerochaete chrysosporium,C was inoculated with non-immobilized Phanerochaete chrysosporium,and D was only with pentachlorophenol(PCP)-contaminated soils open to air.By contrastive analyses,the feasibility of applying composting to the bioremediation of the PCP-contaminated soil was discussed.It can be seen from the experimental results that composting with inocula was better than that without innoculation and after 60d composting,more than 94% PCP in the compost was degraded;meanwhile the effect by immobilized fungi was better than that by nonimmobilized one.From the experimental data it shows that the PCP degradation achieved 50% on 9d by immobilized fungi.What's more,shown by indicators of germination index,volatile solids,microbial carbon activity and other factors,the compost were mature and of no hazard to plant at the end,which provided the benign environment for the Phanerochaete chrysosporium to degrade the PCP so that the bioremediation and composting could be combined together.