| 木质素降解菌的分离鉴定及木素过氧化物酶的纯化 |
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| 引用本文： | 杨金水,刘葳,倪晋仁.木质素降解菌的分离鉴定及木素过氧化物酶的纯化[J].环境科学,2006,27(5):981-985. |
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| 作者姓名： | 杨金水 刘葳 倪晋仁 |
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| 作者单位： | 北京大学环境工程系,水沙科学教育部重点实验室,北京,100871 |
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| 基金项目： | 中国博士后科学基金项目(2005037279) |
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| 摘 要： | 采用苯胺蓝脱色法,从活性污泥中分离筛选得到2株具有分泌木质素降解过氧化物酶能力的细菌菌株PKE117和PKE225.根据生理生化反应结果和16SrDNA序列分析,初步确定菌株PKE117为Pseudomanas属的1个新种,PKE225为Pseudomanas thermaerum的1个新菌株.对于菌株PKE117的发酵液依次经DEAE-纤维素32、Sephadex G-75凝胶过滤纯化,木素过氧化物酶的藜芦醇氧化比活力从0.87U/mg提高到204.5U/mg,酶的纯化倍数为235.1,回收率15%.
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| 关 键 词： | 木质素 分离鉴定 木素过氧化物酶 纯化 |
| 文章编号： | 0250-3301（2006）05-0981-05 |
| 收稿时间： | 2005-05-30 |
| 修稿时间： | 2005-05-302005-08-25 |
Isolation,Identification of Lignin-Degrading Bacteria and Purification of Lignin Peroxidase |
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| YANG Jin-shui,LIU Wei and NI Jin-ren.Isolation,Identification of Lignin-Degrading Bacteria and Purification of Lignin Peroxidase[J].Chinese Journal of Environmental Science,2006,27(5):981-985. |
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| Authors: | YANG Jin-shui LIU Wei and NI Jin-ren |
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| Institution: | The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing 100871,China |
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| Abstract: | Two strains that could use lignin as sole carbon source and excrete peroxidases were isolated from activated sludge.Both strains are belonged to Pseudomanas based on morphological,physio-biochenical characterizatics and homology identification of 16S rDNA sequence,in which strain PKE117 is identified as a new species,while the strain PKE225 is identified as Pseudomanas thermaerum.Crude enzyme in liquid fermentation of PKE117 was analyzed with DEAE-cellulose 32 ion-exchanger resin chromatography and Sephadex G-75 gel-filtration in proper order.The lignin peroxidase specific activity increases from 0.87U/mg to 204.5U/mg,purification multiple is 235.1 and callback rate is 15%. |
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| Keywords: | lignin isolation and identification lignin peroxidase purification |
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