Alcaligenes sp. XJ-T-1利用废弃油脂生产破乳剂研究

生物破乳剂是一种应用于油水乳状液分离的新型破乳剂.本研究对筛选得到的1株高效破乳剂产生菌XJ-T-1（鉴定结果为Alcaligenes sp.）代谢产生的生物破乳剂进行了理化性质、破乳能力和废弃油脂利用能力分析.采用废弃油脂Ⅱ为碳源，可提高生物破乳剂产量4.6倍，XJ-T-1在以液体石蜡和废弃油脂培养8　d后该菌能使水的表面张力从72 mN/m下降到32 mN/m， CMC-1分别为10、 20；以液体石蜡为碳源培养得到的生物破乳剂在油包水型、水包油型模型乳状液的破乳率分别达到了96%、 50%；而以废弃油脂Ⅱ为碳源培养得到的生物破乳剂的破乳率分别为97.8%、 65％；采用煤油脱出率、乳状液脱除率和水脱出率3种评价方式对破乳效果评价发现生物破乳剂最先作用于乳状液的连续相；采用TLC和IR对废弃油脂生产破乳剂得到的破乳剂有效成分进行鉴定，结果为脂肽与石蜡培养产物相同.

Production of Biodemulsifier by Alcaligenes sp. XJ-T-1 with Waste Frying Oil

As a new member of demulsifier family,biodemulsifier is applied in oil-water emulsion breaking.A strain,XJ-T-1,was isolated from petroleum-contaminated soil and identified as Alcaligenes sp..Its physicochemical properties,demulsification capability and utilization of waste oil were further investigated.With waste frying oil(WFO) as carbon source,the demulsifier produced by Alcaligenes sp.showed high demulsifying capability.Moreover,the production of demulsifier was 4.6 times of that generated with paraffine...