| 热碱-分步酶水解-厌氧消化工艺处理秸秆畜粪混合物料及其甲烷高值化条件 |
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| 引用本文： | 卞爱琴,远野,张璐璐,付强,陈天明,何磊,丁成,王爱杰.热碱-分步酶水解-厌氧消化工艺处理秸秆畜粪混合物料及其甲烷高值化条件[J].环境科学,2019,40(2):1003-1010. |
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| 作者姓名： | 卞爱琴 远野 张璐璐 付强 陈天明 何磊 丁成 王爱杰 |
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| 作者单位： | 盐城工学院环境科学与工程学院,盐城,224051;盐城工学院环境科学与工程学院, 盐城 224051;中国科学院生态环境研究中心环境生物技术重点实验室,北京 100085;江苏科易达环保科技有限公司,盐城,224007 |
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| 基金项目： | 国家自然科学基金项目（51608467）；盐城市农业科技创新专项引导资金项目（yk2016030）；江苏省普通高校研究生科研创新计划项目（SJCX17-YG02） |
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| 摘 要： | 为获得秸秆畜粪混合物料在厌氧消化过程中的甲烷高值化产出,提出了一种新型"热碱-分步酶水解-厌氧消化"组合工艺.以玉米秸秆和牛粪混合物料作为实验对象,考察物料中纤维素、半纤维素、蛋白质获得高溶出效率的热碱预处理条件;分步投加纤维素酶和蛋白酶的剂量及水解时间;热碱预处理后的混合浆液和热碱-酶水解后的混合水解液厌氧消化甲烷产率及产气周期.结果表明,在80℃和0. 5%Na OH碱用量条件下,纤维素、半纤维素和蛋白质的溶出效率(%TS)最高,与未预处理相比(对照组),分别提高24. 84%、12. 24%和8. 92%;分步酶水解的过程和条件为:先投加80 U·g-1的纤维素酶水解18 h,再投加20 U·g-1的蛋白酶水解4 h,纤维素和蛋白质的水解效率可分别达到74. 08%和74. 01%,获得的水解液中糖类提高12～32倍;在厌氧消化阶段,热碱-酶水解后的水解液甲烷产量最高值可达750 m L·h-1,产气周期50 h,相比于热碱预处理后的底物消化(对照组),产甲烷效率提高了约14倍,产气周期缩短了约17 d.热碱-酶水解预处理能有效地解除混合物料厌氧消化过程的水解限速,研究结果可以为开发高效的农业废弃物能源高值化利用技术提供参考依据.
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| 关 键 词： | 秸秆畜粪 混合物料 热碱预处理 酶水解 厌氧消化 甲烷产率 |
| 收稿时间： | 2018/7/13 0:00:00 |
| 修稿时间： | 2018/8/1 0:00:00 |
Performance of Treating Straw and Animal Manure Mixture by an Integrated Process of Thermo-alkali-bi-enzyme Hydrolysis-anaerobic Digestion and Conditions of High Methane Yield |
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| BIAN Ai-qin,YUAN Ye,ZHANG Lu-lu,FU Qiang,CHEN Tian-ming,HE Lei,DIND Cheng and WANG Ai-jie.Performance of Treating Straw and Animal Manure Mixture by an Integrated Process of Thermo-alkali-bi-enzyme Hydrolysis-anaerobic Digestion and Conditions of High Methane Yield[J].Chinese Journal of Environmental Science,2019,40(2):1003-1010. |
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| Authors: | BIAN Ai-qin YUAN Ye ZHANG Lu-lu FU Qiang CHEN Tian-ming HE Lei DIND Cheng and WANG Ai-jie |
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| Institution: | School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China,School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China;Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,Jiangsu Keyida Environmental Science and Technology Co., Ltd., Yancheng 224007, China,School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China,School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China,School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China,School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China and School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China;Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
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| Abstract: | |
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| Keywords: | straw and animal manure mixture thermo-alkali pretreatment enzyme hydrolysis anaerobic digestion methane yield |
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