| 可变电荷与恒电荷土壤胶体对DNA吸附与解吸特征 |
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| 引用本文： | 王代长,王慎阳,蒋新,衡利沙,谭金芳,刘世亮,曹永鲜.可变电荷与恒电荷土壤胶体对DNA吸附与解吸特征[J].环境科学,2009,30(9):2761-2766. |
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| 作者姓名： | 王代长 王慎阳 蒋新 衡利沙 谭金芳 刘世亮 曹永鲜 |
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| 作者单位： | 河南农业大学资源与环境学院;中国科学院南京土壤研究所; |
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| 基金项目： | 国家自然科学基金项目(40571082);;国家杰出青年科学基金项目(40325001);;河南农业大学创新基金项目(2006-CX-017) |
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| 摘 要： | 采用平衡法研究了红壤胶体、砖红壤胶体、潮土胶体和褐土胶体在不同pH值条件下DNA吸附与解吸特征.结果表明,在NaCl和KCl电解质体系中,4种土壤胶体在吸附过DNA后,溶液pH值均有不同程度的增加,pH增加的幅度为红壤胶体>砖红壤胶体>潮土胶体>褐土胶体;NaCl电解质体系>KCl电解质体系.土壤胶体对DNA的吸附量均随着pH值的升高而降低,在pH为2~4,不同胶体对DNA的吸附量保持最大值,约为13.1~14.8μg.mg-1.当平衡溶液pH值从4.2开始上升至8.6,在NaCl体系中,砖红壤胶体和红壤胶体上DNA的吸附量下降幅度约5.5μg.mg-1,而在KCl体系中,DNA的吸附量下降幅度约2.1μg.mg-1.2种电解质体系,潮土胶体与褐土胶体上DNA的吸附量下降幅度约为8.3~12.2μg.mg-1.DNA吸附量下降幅度为恒电荷土壤(潮土和褐土)胶体>可变电荷土壤(红壤和砖红壤)胶体.用NaOAc和NaH2PO4对土壤胶体吸附DNA的解吸时,可变电荷土壤胶体与恒电荷土壤胶体解吸规律有明显差异.在3种溶液pH值为3、5和7时,可变电荷土壤(红壤和砖红壤)胶体上NaOAc解吸率约10%~24.5%,Na...
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| 关 键 词： | 土壤胶体 DNA pH值 吸附与解吸 可变电荷与恒电荷 |
| 收稿时间： | 2008/9/16 0:00:00 |
| 修稿时间： | 2009/2/26 0:00:00 |
Characteristics of DNA Adsorption and Desorption in Variable and Constant Charge Soil Colloids |
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| WANG Dai-zhang,WANG Shen-yang,JIANG Xin,HENG Li-sh,TAN Jin-fang,LIU Shi-liang and CAO Yong-xian.Characteristics of DNA Adsorption and Desorption in Variable and Constant Charge Soil Colloids[J].Chinese Journal of Environmental Science,2009,30(9):2761-2766. |
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| Authors: | WANG Dai-zhang WANG Shen-yang JIANG Xin HENG Li-sh TAN Jin-fang LIU Shi-liang and CAO Yong-xian |
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| Institution: | 1.Colloge of Resources and Environment;Henan Agricultural University;Zhengzhou 450002;China;2.Institute of Soil Science;Chinese Academy of Sciences;Nanjing 210008;China |
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| Abstract: | The characteristics of adsorption and desorption of DNA by Red soil colloid,Latosol colloid,Chao colloid and Cinnamon colloid at different pH values were studied using a batch method.It showed that there was an increase of solution pH after adsorption of DNA by the four soil colloids in both NaCl and KCl electrolyte systems.The increasing ranges of pH values were in order of Red soil colloid>Latosol colloid>Chao colloid>Cinnamon colloid,and NaCl electrolyte system>KCl electrolyte system.The amounts of DNA a... |
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| Keywords: | soil colloids DNA pH value adsorption and desorption variable and constant charge |
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