硝基苯污染底质的微生物强化修复研究

采用从污染底质中分离出的可降解硝基苯的恶臭假单胞菌,对硝基苯污染底质的微生物强化修复进行了实验室和现场实验研究.该细菌在未灭菌的河水中可以硝基苯为唯一碳源生长,低温条件下(5℃),对于100g的含有11.8mg/kg硝基苯的污染底质,投加2mL(107cells/mL)菌液可以在4d完全降解底质中的硝基苯,实现对污染底质的强化修复.该过程中无须投加额外的氮、磷及其他的营养盐,说明污染底质中含有足够的细菌生长所需的营养物质.在使用河水和底质的现场实验中,当底质和河水中的硝基苯初始浓度在7～8mg/kg,50～61mg/L之间时,投加硝基苯降解菌可使底质和河水中硝基苯的降解时间缩短了40h以上,河水中的硝基苯先于底质中的硝基苯被细菌所降解.

Bioremediation of Nitrobenzene-polluted Sediments Using Pseudomonas putida

Bioremediation of nitrobenzene-polluted sediments was studied through lab-scale and in situ experiments. The polluted sediments were remediated through the addition of bacterial separated from the sediments, even at a low temperature of 5 degrees C. Nitrobenzene at a concentration of 11.8 mg/kg was biodegraded within 4 d with the addition of 2 mL cell solution (10(7) cells/mL). No extra nutrients were needed for the bioremediation process, showing that enough nutrients existed in the sediments. For the in situ bioremediation experiment, the initial nitrobenzene concentrations at solution and sediments were 50-61 mg/L and 7-8 mg/L respectively. The remediation process was also enhanced through cell addition. The above mentioned nitrobenzene was biodegraded within 48 h, compared with 96 h without cells presence. Nitrobenzene in solution was removed preferentially than those in sediments.