固定化蛋白酶对废水中蛋白质的水解效果与机理

采用吸附方法将蛋白酶固定于活性炭、大孔树脂和硅胶载体上,分别研究了3种载体蛋白酶固定化效果,酶活性以及对淀粉加工黄浆废水中蛋白质的水解效果.批次试验结果表明,3种载体蛋白酶固定效果分别为:硅胶20.31%,大孔树脂37.85%,活性炭61.95%;酶活性最高时pH均为5.5;酶活性分别为3.6mg/min、21.48mg/min和18.68mg/min .水解后,大孔树脂和活性炭固定酶的水解产物氨基酸总量分别较水解前增加21.26倍和14.6倍;产物中检出1 6种氨基酸,较水解前增加2种.以大孔树脂为载体构建固定化酶反应器进行连续进水试验,结果表明:进水中蛋白质浓度明显影响反应器的水解效果,当其蛋白质浓度与反应器的酶浓度匹配时(500mg/L) ,反应器能获得80 %蛋白质水解率和36.1%有机物水解酸化率.挥发性水解产物组成以乙醇和乙酸为主,分别占50.3%,33.3%.为乙醇型水解.

Hydrolysis and Mechanism of Wastewater Contained Protein Using Immobilized Protein Enzyme

Protein-enzyme was immobilized on big-hole resin,activated charcoal and silica particles by a absorption method.This study focuses on effect of enzyme immobilization,thermal stability and hydrolysis efficiency of wastewater.The result of batch test show that the ratio of immobilized enzyme on activated charcoal,big-hole resin and silica particles was 61.95%,37.85% and 20.31% respectively.The highest of thermal ability of activated charcoal,big-hole resin and silica particles was 18.68mg/min,21.48mg/min and 3.6mg/min respectively at pH 5.5.The total amino-acids of big-hole resin and activated charcoal immobilized enzyme after hydrolysis was respectively increased to 21.26 times and 14.6 times. The kind of amino-acid was increased from 14 to 16 kinds. The continuous-flow test result indicate that concentration of substrate in influent had significant effect on the hydrolysis rate of immobilized enzyme reactor.The reactor was able to gain hydrolysis rate of 80% for protein and 36.1%(VFA/COD%) for organic materials in the wastewater with a moderate concentration of protein (500mg/L).The ethanol and acetic of volatile hydrolysis-mixture composition were 50.3% and 33.3% respectively.