| 分层型水库水体好氧不产氧光合细菌时空演替特征 |
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| 引用本文： | 张海涵,王燕,黄廷林,王晨旭,路林超,司凡,李楠,刘凯文,闫苗苗,苗雨甜.分层型水库水体好氧不产氧光合细菌时空演替特征[J].环境科学,2020,41(5):2188-2197. |
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| 作者姓名： | 张海涵 王燕 黄廷林 王晨旭 路林超 司凡 李楠 刘凯文 闫苗苗 苗雨甜 |
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| 作者单位： | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055,西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室,西安 710055 |
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| 基金项目： | 国家自然科学基金项目（51978561，51979217）；陕西省重点研发计划项目（2018ZDXM-SF-020，2018KW-011） |
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| 摘 要： | 好氧不产氧光合细菌（aerobic anoxygenic photosynthesis bacteria，AAPB）以多样的群落结构及独特的代谢功能在水体物质循环中扮演着重要角色.基于实时荧光定量PCR及Illumina MiSeq高通量DNA测序技术研究金盆水库水体中AAPB丰度及群落结构时空演替特征，结合冗余分析揭示环境因子对其群落结构影响规律.结果表明，金盆水库水体AAPB丰度（以pufM基因计）变化范围为（6.70±0.43）×103~（2.69±0.15）×104 copies·mL-1，最大值出现于10月，且随水深增加而减小.样本主要归为19个属（除未分类菌属外），优势AAPB菌属包括慢生根瘤菌属（Bradyrhizobium sp.）和甲烷菌属（Methylobacterium sp.），两者隶属α-变形菌（α-Proteobacteria），其中慢生根瘤菌属（Bradyrhizobium sp.）占比于11月最高，高达60%以上（除10 m外），此外也发现了以低比例存在的红长命菌属（Rubrivivax sp.），隶属β-变形菌（β-Proteobacteria）.AAPB不同属间存在较强的互作关系，如红杆菌属（Rhodobacter sp.）与小红卵菌属（Rhodovulum sp.）呈正相关，噬氢菌属（Hydrogenophaga sp.）与慢生根瘤菌属（Bradyrhizobium sp.）呈负相关等.AAPB种群结构组成及分布差异显著，主要受T、TN、NO3--N和光照强度影响，且由环境因素综合调控.如10月水深0、5和15 m水体AAPB种群结构受水温（T）、总氮（TN）和总磷（TP）显著影响，12月水深5 m水体AAPB种群结构受光照强度、pH、溶解氧（DO）和叶绿素a（Chla）显著影响等.研究结果对解析分层型水库水体AAPB丰度和多样性的时空变化特征具有指导意义，并为探索AAPB种群结构的水环境驱动因素提供理论依据.
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| 关 键 词： | 分层型水库 好氧不产氧光合细菌(AAPB) pufM基因 qPCR 高通量测序 种群结构 |
| 收稿时间： | 2019/10/9 0:00:00 |
| 修稿时间： | 2019/12/5 0:00:00 |
Spatial and Temporal Succession Characteristics of Aerobic Anoxygenic Photosynthesis Bacteria in a Stratified Reservoir |
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| ZHANG Hai-han,WANG Yan,HUANG Ting-lin,WANG Chen-xu,LU Lin-chao,SI Fan,LI Nan,LIU Kai-wen,YAN Miao-miao and MIAO Yu-tian.Spatial and Temporal Succession Characteristics of Aerobic Anoxygenic Photosynthesis Bacteria in a Stratified Reservoir[J].Chinese Journal of Environmental Science,2020,41(5):2188-2197. |
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| Authors: | ZHANG Hai-han WANG Yan HUANG Ting-lin WANG Chen-xu LU Lin-chao SI Fan LI Nan LIU Kai-wen YAN Miao-miao and MIAO Yu-tian |
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| Institution: | Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China,Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China and Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi''an University of Architecture and Technology, Xi''an 710055, China |
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| Abstract: | |
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| Keywords: | stratified reservoir aerobic anoxygenic photosynthesis bacteria (AAPB) pufM gene qPCR high-throughput sequencing community structure |
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