| ITS高通量测序研究黄海微型真核浮游生物多样性及分布特征 |
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| 引用本文： | 张莉,林佳宁,张远,王书平,张晓娇.ITS高通量测序研究黄海微型真核浮游生物多样性及分布特征[J].环境科学,2018,39(5):2368-2379. |
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| 作者姓名： | 张莉 林佳宁 张远 王书平 张晓娇 |
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| 作者单位： | 辽宁大学环境学院;中国环境科学研究院环境基准与风险评估国家重点实验室;中国环境科学研究院流域水生态保护技术研究室;大连海洋大学水产与生命学院 |
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| 基金项目： | 国家自然科学基金项目（41571050） |
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| 摘 要： | 微型真核浮游生物是海洋生物群落的重要组成部分,对维持海洋生态系统的稳定发挥着重要的作用.本研究对黄海水环境样本的标记基因ITS进行Illumina高通量测序,探讨了黄海微型真核浮游生物的多样性及群落结构特征,并分析了其与环境因子之间的关系.结果表明:(1)基于标记基因ITS的高通量测序技术可获得较丰富的真菌、浮游植物、浮游动物种类,其中真菌OTUs浮游植物OTUs浮游动物OTUs,真菌reads数浮游动物reads数浮游植物reads数.(2)不同水域微型浮游生物的物种丰度及多样性程度不同,Ace指数及Chao1指数表现为南黄海北黄海东海北部冷水团,Simpson指数及Shannon-Wiener指数表现为东海北部北黄海南黄海冷水团,冷水团水域的物种丰度及多样性最低.(3)随着温度的升高,真菌、浮游植物、浮游动物reads数、OTUs均呈现出上升的趋势;随着盐度的增加,真菌的reads数、OTUs升高,而浮游植物、浮游动物的reads数、OTUs下降,其中浮游植物的reads数、OTUs显著下降(P0.05);在北纬30°~40°范围内,随着纬度的升高,真菌、浮游动物的Shannon-Wiener指数上升,而浮游植物Shannon-Wiener指数下降;与海岸线的距离越大,真菌、浮游植物、浮游动物Shannon-Wiener指数越低.本研究首次以ITS为扩增子高通量测序研究海洋微型真核浮游生物,丰富了微型真核浮游生物的认识,揭示了高通量测序技术在该海域研究的可行性,为其他海域微型真核浮游生物的研究提供了借鉴.
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| 关 键 词： | 黄海 微型浮游生物 高通量测序技术 内转录间隔区(ITS) 多样性 |
| 收稿时间： | 2017/10/10 0:00:00 |
| 修稿时间： | 2017/11/10 0:00:00 |
Eukaryotic Micro-plankton Community Diversity and Characteristics of Regional Distribution in the Yellow Sea by ITS High-throughput Sequencing |
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| ZHANG Li,LIN Jia-ning,ZHANG Yuan,WANG Shu-ping and ZHANG Xiao-jiao.Eukaryotic Micro-plankton Community Diversity and Characteristics of Regional Distribution in the Yellow Sea by ITS High-throughput Sequencing[J].Chinese Journal of Environmental Science,2018,39(5):2368-2379. |
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| Authors: | ZHANG Li LIN Jia-ning ZHANG Yuan WANG Shu-ping and ZHANG Xiao-jiao |
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| Institution: | College of Environmental Sciences, Liaoning University, Shenyang 110036, China;State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;Laboratory of Riverine Ecological Conservation and Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China,State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;Laboratory of Riverine Ecological Conservation and Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China,State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;Laboratory of Riverine Ecological Conservation and Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China,State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;Laboratory of Riverine Ecological Conservation and Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China and State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;Laboratory of Riverine Ecological Conservation and Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China |
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| Abstract: | |
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| Keywords: | Yellow Sea micro-plankton high-throughput sequencing technology internal transcribed space (ITS) diversity |
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