| 东太湖水产养殖对沉积物中氨氧化原核生物的影响 |
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| 引用本文： | 储瑜,何肖微,曾巾,赵大勇,孙强,曹萍,吴庆龙.东太湖水产养殖对沉积物中氨氧化原核生物的影响[J].环境科学,2018,39(9):4206-4214. |
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| 作者姓名： | 储瑜 何肖微 曾巾 赵大勇 孙强 曹萍 吴庆龙 |
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| 作者单位： | 河海大学水文水资源与水利工程科学国家重点实验室;中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室;江苏省太湖渔业管理委员会办公室 |
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| 基金项目： | 国家科技支撑计划项目（2015BAD13B01，2015BAD13B00） |
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| 摘 要： | 本研究旨在了解东太湖水产养殖区表层沉积物中氨氧化微生物的群落特征.以编码氨单加氧酶的α亚基(amo A)基因为标记,通过实时荧光定量PCR技术(real-time q PCR)分析环境中好氧氨氧化原核生物的丰度;通过构建克隆文库、测序,进而划分操作分类单元(OTUs)构建系统发育树,分析氨氧化微生物的群落结构与多样性.分析养殖区和对照区表层沉积物中氨氧化微生物群落结构和多样性发现,养殖区中氨氧化细菌(ammonia-oxidizing bacteria,AOB)的丰度更高而对照区中氨氧化古菌(ammonia-oxidizing archaea,AOA)的丰度更高;AOA在养殖区多样性更高,AOB在对照区多样性更高;AOA在养殖区和对照区沉积物中的优势类群均为Nitrosopumilus,AOB在养殖区和对照区沉积物中的优势类群相同均为亚硝化螺旋菌属(Nitrosospira).水产养殖主要通过影响沉积物中的氨氮含量来影响AOA和AOB的丰度,养殖过程会影响AOA的群落结构而对AOB的群落结构则无明显影响.
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| 关 键 词： | 东太湖 水产养殖 沉积物 氨氧化细菌 氨氧化古菌 |
| 收稿时间： | 2017/11/13 0:00:00 |
| 修稿时间： | 2018/3/13 0:00:00 |
Effects of Aquaculture on Ammonia-oxidizing Prokaryotes in Sediments of Eastern Lake Taihu |
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| CHU Yu,HE Xiao-wei,ZENG Jin,ZHAO Da-yong,SUN Qiang,CAO Ping and WU Qing-long.Effects of Aquaculture on Ammonia-oxidizing Prokaryotes in Sediments of Eastern Lake Taihu[J].Chinese Journal of Environmental Science,2018,39(9):4206-4214. |
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| Authors: | CHU Yu HE Xiao-wei ZENG Jin ZHAO Da-yong SUN Qiang CAO Ping and WU Qing-long |
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| Institution: | State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China,State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China,State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China,State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China,Taihu Fishery Management Committee Office, Suzhou 215004, China,Taihu Fishery Management Committee Office, Suzhou 215004, China and State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China |
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| Abstract: | |
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| Keywords: | Eastern Lake Taihu aquaculture sediment ammonia-oxidizing bacteria ammonia-oxidizing archaea |
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